H Park1, S Shin1, M H Park1, Y S Kim2, C Ahn2, J Ha3, E Y Song4. 1. Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea. 2. Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea. 3. Department of Surgery, Seoul National University College of Medicine, Seoul, Korea. 4. Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea. Electronic address: eysong1@snu.ac.kr.
Abstract
OBJECTIVE: Although interleukin 17 (IL-17) has some roles in renal transplantation, the influence of IL-17 gene single-nucleotide polymorphisms (SNPs) on renal transplantation has not been studied. METHODS: The associations of 5 IL-17F gene SNPs (-1507G/A, 6329G/A, 7384A/G, 7470G/A, and 7489A/G) with renal transplantation outcome were analyzed. Polymerase chain reaction with sequence-specific primers (for -1507G/A and 6329G/A) and direct sequencing (for 7384A/G, 7470G/A, and 7489A/G) were performed on 282 renal transplantation recipients and 210 healthy controls. RESULTS: IL-17F SNPs were not associated with acute rejection. Recipients with G allele on 7489A/G showed lower graft survival than recipients without G allele (P = .04). In multivariate analysis, G allele on 7489A/G was an independent risk factor for graft failure (odds ratio = 2.77, P = .03). CONCLUSION: IL-17F gene SNP 7489A/G was associated with renal graft failure. Further studies are needed in larger number of patients.
OBJECTIVE: Although interleukin 17 (IL-17) has some roles in renal transplantation, the influence of IL-17 gene single-nucleotide polymorphisms (SNPs) on renal transplantation has not been studied. METHODS: The associations of 5 IL-17F gene SNPs (-1507G/A, 6329G/A, 7384A/G, 7470G/A, and 7489A/G) with renal transplantation outcome were analyzed. Polymerase chain reaction with sequence-specific primers (for -1507G/A and 6329G/A) and direct sequencing (for 7384A/G, 7470G/A, and 7489A/G) were performed on 282 renal transplantation recipients and 210 healthy controls. RESULTS:IL-17F SNPs were not associated with acute rejection. Recipients with G allele on 7489A/G showed lower graft survival than recipients without G allele (P = .04). In multivariate analysis, G allele on 7489A/G was an independent risk factor for graft failure (odds ratio = 2.77, P = .03). CONCLUSION:IL-17F gene SNP 7489A/G was associated with renal graft failure. Further studies are needed in larger number of patients.
Authors: Hyewon Park; Nuri Lee; Ji Won In; Eun Youn Roh; Kyoung Un Park; Sue Shin; Jaeseok Yang; Eun Young Song Journal: Ann Lab Med Date: 2017-09 Impact factor: 3.464