Draft Genome Sequence of Clostridium ultunense Strain BS (DSMZ 10521), Recovered from a Mixed Culture.

Clostridium ultunense BS is the first isolated strain (type strain) of C. ultunense that was identified as a mesophilic syntrophic acetate-oxidizing bacterium (SAOB). Here, we report the draft genome sequence of this strain, which will help us to elucidate the mechanism of syntrophic acetate oxidization.

GENOME ANNOUNCEMENT

Clostridium ultunense BS was isolated from a triculture enriched from a laboratory-scale digester (1). It is the type strain of C. ultunense and shows 99% 16S rRNA gene identity with another strain, Esp, the draft genome sequence of which was published recently (2, 3). Most of our knowledge about C. ultunense is based on investigation of this type strain (4). C. ultunense BS is Gram-positive, spore-forming, and rod-shaped. It can oxidize acetate to H2 and CO2 when cocultured with hydrogenotrophic methanogen (1). C. ultunense BS can also live in pure culture and ferment many kinds of simple organic matter and produce acetate as a main product (1). Dense cell suspensions of this strain can convert H2 and CO2 to acetate, which suggests that it is an acetogen (1). Furthermore, some enzyme activities of the Wood-Ljungdahl pathway were detected in this strain, which supports the hypothesis that it oxidizes acetate by the reverse Wood-Ljungdahl pathway (4). C. ultunense BS was originally deposited in the German Collection of Microorganisms and Cell Cultures (DSMZ) as DSM 10521 (1). However, this strain was found to be contaminated with another strain (Aminobacterium colombiense) based on 16S rRNA analysis by our lab, which was also confirmed by the DSMZ (Stefan Spring, personal communication). In order to obtain the genome of C. ultunense BS, we sequenced this mixed culture. The genome data of A. colombiense strain ALA-1T (=DSM 12261) was used to filter A. colombiense sequences and scrutinize the C. ultunense assembly. Sequencing the genome of C. ultunense BS, and comparing it to the genomes of other syntrophic acetate-oxidizing bacterial (SAOB) strains, including Tepidanaerobacter acetatoxydans strain Re1, C. ultunense strain Esp, and Thermacetogenium phaeum, might give insight into the mechanism of syntrophic acetate oxidization (3, 5, 6).

The C. ultunense BS genome was sequenced using both the Roche 454 GS FLX+ system and the Illumina GAIIx system. Newbler assembler version 2.8 was used to assemble the 454 reads into contigs, and SSPACE was used to extend and scaffold the preassembled contigs (7). The scaffolds belonging to A. colombiense were filtered with the genome of A. colombiense ALA-1T using MUMmer (8, 9). The C. ultunense BS genome was assembled into 12 scaffolds, for a total length of 3,216,692 bp. The average length of the scaffolds is 268 kb. The final coverage for the C. ultunense BS genome is ~85× for 454 reads. In total, 3,242 open reading frames (ORFs) were predicted by GeneMark (10). In addition, 64 tRNAs were predicted using tRNAscan-SE 1.23 (11), and four copies of 5S rRNA and one copy each of 16S rRNA and 23S rRNA were identified with RNAmmer 1.2 (12). The genome of C. ultunense BS has a G+C content of 31.5%. Further analysis that compares the genomes of C. ultunense BS and other SAOB strains is in progress, and the detailed results will be published in future.

Nucleotide sequence accession numbers.

The draft genome sequence of C. ultunense BS has been deposited in the GenBank database under the accession no. AZSU00000000. The version described in this paper is the first version, AZSU01000000.