Literature DB >> 24502799

Comparative analysis of mitosis-specific antibodies for bulk purification of mitotic populations by fluorescence-activated cell sorting.

Amy E Campbell1,2, Chris C-S Hsiung1,2, Gerd A Blobel1,2.   

Abstract

Mitosis entails complex chromatin changes that have garnered increasing interest from biologists who study genome structure and regulation-fields that are being advanced by high-throughput sequencing (Seq) technologies. The application of these technologies to study the mitotic genome requires large numbers of highly pure mitotic cells, with minimal contamination from interphase cells, to ensure accurate measurement of phenomena specific to mitosis. Here, we optimized a fluorescence-activated cell sorting (FACS)-based method for isolating formaldehyde-fixed mitotic cells--at virtually 100% mitotic purity and in quantities sufficient for high-throughput genomic studies. We compared several commercially available antibodies that react with mitosis-specific epitopes over a range of concentrations and cell numbers, finding antibody MPM2 to be the most robust and cost-effective.

Entities:  

Keywords:  FACS; H3S10ph; MPM2; Mitosis

Mesh：

Substances：

Year:  2014        PMID： 24502799      PMCID： PMC4090248          DOI： 10.2144/000114137

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  13 in total

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Authors:  J H TAYLOR
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5.  Bookmarking by specific and nonspecific binding of FoxA1 pioneer factor to mitotic chromosomes.

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6.  Cloning of cDNAs for M-phase phosphoproteins recognized by the MPM2 monoclonal antibody and determination of the phosphorylated epitope.

Authors:  J M Westendorf; P N Rao; L Gerace
Journal:  Proc Natl Acad Sci U S A       Date:  1994-01-18       Impact factor: 11.205

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Authors:  M J Hendzel; Y Wei; M A Mancini; A Van Hooser; T Ranalli; B R Brinkley; D P Bazett-Jones; C D Allis
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9.  Monoclonal antibodies to mitotic cells.

Authors:  F M Davis; T Y Tsao; S K Fowler; P N Rao
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10.  Mitotic bookmarking by transcription factors.

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Authors:  Chris C-S Hsiung; Christapher S Morrissey; Maheshi Udugama; Christopher L Frank; Cheryl A Keller; Songjoon Baek; Belinda Giardine; Gregory E Crawford; Myong-Hee Sung; Ross C Hardison; Gerd A Blobel
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3.  A hyperactive transcriptional state marks genome reactivation at the mitosis-G1 transition.

Authors:  Chris C-S Hsiung; Caroline R Bartman; Peng Huang; Paul Ginart; Aaron J Stonestrom; Cheryl A Keller; Carolyne Face; Kristen S Jahn; Perry Evans; Laavanya Sankaranarayanan; Belinda Giardine; Ross C Hardison; Arjun Raj; Gerd A Blobel
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4.  USP11 deubiquitinates RAE1 and plays a key role in bipolar spindle formation.

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6.  Mitotic catenation is monitored and resolved by a PKCε-regulated pathway.

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7.  RCBTB1 Deletion Is Associated with Metastatic Outcome and Contributes to Docetaxel Resistance in Nontranslocation-Related Pleomorphic Sarcomas.

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  7 in total

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