Literature DB >> 24499941

Knockin' on pHeaven's door: a fast and reliable high-throughput compatible zero-background cloning procedure.

Quirino Schefer1, Sandy Hallmann, Carsten Grötzinger.   

Abstract

We created a pair of vectors allowing simple and efficient molecular cloning of any gene of interest with minimal consumption of time, labor and material. This system is applicable for standard molecular cloning, high-throughput cloning and generation of fusion protein libraries as well as for more complex gene assembly purposes. Also, this zero-background procedure allows going from cDNA to gene expression analysis in a defined vector in <2 days.

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Year:  2014        PMID： 24499941     DOI： 10.1007/s12033-014-9736-2

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  19 in total

1.  Enzyme-free cloning: a rapid method to clone PCR products independent of vector restriction enzyme sites.

Authors:  D Tillett; B A Neilan
Journal:  Nucleic Acids Res       Date:  1999-10-01       Impact factor: 16.971

2.  DNA cloning using in vitro site-specific recombination.

Authors:  J L Hartley; G F Temple; M A Brasch
Journal:  Genome Res       Date:  2000-11       Impact factor: 9.043

3.  Ligation-independent cloning of PCR products (LIC-PCR).

Authors:  C Aslanidis; P J de Jong
Journal:  Nucleic Acids Res       Date:  1990-10-25       Impact factor: 16.971

4.  Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products.

Authors:  D Marchuk; M Drumm; A Saulino; F S Collins
Journal:  Nucleic Acids Res       Date:  1991-03-11       Impact factor: 16.971

5.  MAGIC, an in vivo genetic method for the rapid construction of recombinant DNA molecules.

Authors:  Mamie Z Li; Stephen J Elledge
Journal:  Nat Genet       Date:  2005-01-30       Impact factor: 38.330

6.  The univector plasmid-fusion system, a method for rapid construction of recombinant DNA without restriction enzymes.

Authors:  Q Liu; M Z Li; D Leibham; D Cortez; S J Elledge
Journal:  Curr Biol       Date:  1998-12-03       Impact factor: 10.834

7.  Assembly of designer TAL effectors by Golden Gate cloning.

Authors:  Ernst Weber; Ramona Gruetzner; Stefan Werner; Carola Engler; Sylvestre Marillonnet
Journal:  PLoS One       Date:  2011-05-19       Impact factor: 3.240

8.  SLiCE: a novel bacterial cell extract-based DNA cloning method.

Authors:  Yongwei Zhang; Uwe Werling; Winfried Edelmann
Journal:  Nucleic Acids Res       Date:  2012-01-12       Impact factor: 16.971

9.  A versatile ligation-independent cloning method suitable for high-throughput expression screening applications.

Authors:  Nick S Berrow; David Alderton; Sarah Sainsbury; Joanne Nettleship; Rene Assenberg; Nahid Rahman; David I Stuart; Raymond J Owens
Journal:  Nucleic Acids Res       Date:  2007-02-22       Impact factor: 16.971

10.  Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments.

Authors:  Hussam H Nour-Eldin; Bjarne G Hansen; Morten H H Nørholm; Jacob K Jensen; Barbara A Halkier
Journal:  Nucleic Acids Res       Date:  2006-09-25       Impact factor: 16.971
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  1 in total

1.  ZeBRα a universal, multi-fragment DNA-assembly-system with minimal hands-on time requirement.

Authors:  David Richter; Katharina Bayer; Thomas Toesko; Stefan Schuster
Journal:  Sci Rep       Date:  2019-02-27       Impact factor: 4.379
  1 in total

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