Hyung Jung Oh1, Bo Young Nam1, Mi Jung Lee1, Chan Ho Kim1, Hyang Mo Koo1, Fa Mee Doh1, Jae Hyun Han1, Eun Jin Kim1, Ji Suk Han1, Jung Tak Park1, Tae-Hyun Yoo1, Shin-Wook Kang2, Dae-Suk Han1, Seung Hyeok Han1. 1. Division of Nephrology, Department of Internal Medicine, College of Medicine, Brain Korea 21, Severance Biomedical Science Institute, Yonsei University, Seoul, Korea. 2. Division of Nephrology, Department of Internal Medicine, College of Medicine, Brain Korea 21, Severance Biomedical Science Institute, Yonsei University, Seoul, Korea Division of Nephrology, Department of Internal Medicine, College of Medicine, Brain Korea 21, Severance Biomedical Science Institute, Yonsei University, Seoul, Korea.
Abstract
INTRODUCTION: It has been reported that klotho deficiency is associated with oxidative stress and inflammation in experimental kidney disease models. Patients with endstage renal disease (ESRD) are particularly characterized by increased oxidative stress and inflammation. However, little is known about the relationship between these features and klotho in patients with ESRD. METHODS: We conducted a single-center, cross-sectional study of 78 patients receiving peritoneal dialysis (PD). Serum concentrations of klotho, high-sensitivity C-reactive protein (hsCRP), interleukin-6 (IL-6), and 8-isoprostane were measured by enzyme-linked immunosorbent assay. To define factors independently associated with klotho, we determined Spearman's correlation coefficients for between co-variates and conducted multiple linear regression analyses. RESULTS: Patients were classified by median concentration of klotho. In patients with klotho levels > 329.6 pg/mL, serum 8-isoprostane and IL-6 levels were significantly higher than in those with klotho levels < 329.6 pg/mL. In correlation analyses, log 8-isoprostane (γ = -0.310, p = 0.006) and log IL-6 (γ = -0.343, p = 0.002) were inversely correlated with log klotho. After adjustment for age, gender, mean arterial pressure, log intact parathyroid hormone, and log IL-6, log 8-isoprostane was independently associated with log klotho (β = -0.158, p = 0.040). However, the significant relationship between klotho and IL-6 was not seen in an adjusted model. CONCLUSIONS: This study showed that circulating klotho levels were significantly associated with 8-isoprostane levels in patients undergoing PD, suggesting a potential link between klotho deficiency and enhanced oxidative stress in ESRD patients.
INTRODUCTION: It has been reported that klotho deficiency is associated with oxidative stress and inflammation in experimental kidney disease models. Patients with endstage renal disease (ESRD) are particularly characterized by increased oxidative stress and inflammation. However, little is known about the relationship between these features and klotho in patients with ESRD. METHODS: We conducted a single-center, cross-sectional study of 78 patients receiving peritoneal dialysis (PD). Serum concentrations of klotho, high-sensitivity C-reactive protein (hsCRP), interleukin-6 (IL-6), and 8-isoprostane were measured by enzyme-linked immunosorbent assay. To define factors independently associated with klotho, we determined Spearman's correlation coefficients for between co-variates and conducted multiple linear regression analyses. RESULTS:Patients were classified by median concentration of klotho. In patients with klotho levels > 329.6 pg/mL, serum 8-isoprostane and IL-6 levels were significantly higher than in those with klotho levels < 329.6 pg/mL. In correlation analyses, log 8-isoprostane (γ = -0.310, p = 0.006) and log IL-6 (γ = -0.343, p = 0.002) were inversely correlated with log klotho. After adjustment for age, gender, mean arterial pressure, log intact parathyroid hormone, and log IL-6, log 8-isoprostane was independently associated with log klotho (β = -0.158, p = 0.040). However, the significant relationship between klotho and IL-6 was not seen in an adjusted model. CONCLUSIONS: This study showed that circulating klotho levels were significantly associated with 8-isoprostane levels in patients undergoing PD, suggesting a potential link between klotho deficiency and enhanced oxidative stress in ESRDpatients.
Authors: Hyoung Rae Kim; Bo Young Nam; Dong Wook Kim; Min Woong Kang; Jae-Hyun Han; Mi Jung Lee; Dong Ho Shin; Fa Mee Doh; Hyang Mo Koo; Kwang Il Ko; Chan Ho Kim; Hyung Jung Oh; Tae-Hyun Yoo; Shin-Wook Kang; Dae Suk Han; Seung Hyeok Han Journal: Am J Kidney Dis Date: 2013-03-27 Impact factor: 8.860
Authors: María C Izquierdo; María V Perez-Gomez; María D Sanchez-Niño; Ana B Sanz; Olga Ruiz-Andres; Jonay Poveda; Juan Antonio Moreno; Jesús Egido; Alberto Ortiz Journal: Nephrol Dial Transplant Date: 2012-12 Impact factor: 5.992
Authors: José M Cóndor; Camila E Rodrigues; Roberto de Sousa Moreira; Daniele Canale; Rildo A Volpini; Maria H M Shimizu; Niels O S Camara; Irene de L Noronha; Lúcia Andrade Journal: Stem Cells Transl Med Date: 2016-06-08 Impact factor: 6.940