A Duran1, H Otiük2, E H Terzi3, M Tosun4, H Oziiü2, T Ocak5, A Kiiüer4. 1. Department of Emergency Medicine, Abant Izzet Baysal University Medical Faculty, Bolu, Turkey. drarifduran@gmail.com 2. Department of Pediatric Surgery, Abant Izzet Baysal University Medical Faculty, Bolu, Turkey. 3. Department of Histology and Embryology, Abant Izzet Baysal University Medical Faculty, Bolu, Turkey. 4. Department of Medical Biochemistry, Abant Izzet Baysal University Medical Faculty, Bolu, Turkey. 5. Department of Emergency Medicine, Abant Izzet Baysal University Medical Faculty, Bolu, Turkey.
Abstract
OBJECTIVE: Ischemia-reperfusion (I-R) injury of the intestine is a significant problem because the initial damage caused by ischemia is exacerbated by reperfusion. In this study, we examined the protective effect of montelukast against I-R-induced intestinal tissue damage. MATERIALS AND METHODS: Eight-week-old male Sprague-Dawley rats were randomly divided into three treatment groups : a sham-operated group, a group receiving I-R, and a group receiving I-R plus montelukast (I-R/M). Tissue samples were evaluated and scored histologically. The blood levels of malondialdehyde (MDA), myeloperoxidase (MPO), glutathione (GSH), and cardiotrophin-1 (CT-1) were measured. RESULTS: In the I-R group, the histological score and the levels of serum MDA and MPO were increased compared with those in the control group. In the I-R/M group, the histological score and serum MDA and MPO levels were significantly decreased compared with those in the I-R group. Additionally, compared with the IR group, the I-R/M group had increased serum GSH and CT-1 levels and a decreased intestinal injury score. Ileal sections from the I-R/M group showed minimal alterations, characterized by moderate lifting of the epithelial layer from the lamina propria, and few apoptotic enterocytes were observed compare with the number in the I-R group. CONCLUSION: The findings of the present study demonstrated that montelukast can protect I-R-induced intestinal damage in rats.
OBJECTIVE:Ischemia-reperfusion (I-R) injury of the intestine is a significant problem because the initial damage caused by ischemia is exacerbated by reperfusion. In this study, we examined the protective effect of montelukast against I-R-induced intestinal tissue damage. MATERIALS AND METHODS: Eight-week-old male Sprague-Dawley rats were randomly divided into three treatment groups : a sham-operated group, a group receiving I-R, and a group receiving I-R plus montelukast (I-R/M). Tissue samples were evaluated and scored histologically. The blood levels of malondialdehyde (MDA), myeloperoxidase (MPO), glutathione (GSH), and cardiotrophin-1 (CT-1) were measured. RESULTS: In the I-R group, the histological score and the levels of serum MDA and MPO were increased compared with those in the control group. In the I-R/M group, the histological score and serum MDA and MPO levels were significantly decreased compared with those in the I-R group. Additionally, compared with the IR group, the I-R/M group had increased serum GSH and CT-1 levels and a decreased intestinal injury score. Ileal sections from the I-R/M group showed minimal alterations, characterized by moderate lifting of the epithelial layer from the lamina propria, and few apoptotic enterocytes were observed compare with the number in the I-R group. CONCLUSION: The findings of the present study demonstrated that montelukast can protect I-R-induced intestinal damage in rats.
Authors: Thomas W Carion; Yuxin Wang; Ashten Stambersky; Abdul Shukkur Ebrahim; Elizabeth A Berger Journal: J Immunol Date: 2022-04-25 Impact factor: 5.426
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