Nucleotide sequence and transcription analysis of the gene coding for subunit III of soybean chloroplast proton-translocating ATPase.

The gene coding for subunit III of CF0-ATPase complex (atpH gene) has been localized on the soybean chloroplast genome by heterologous hybridization with the atpH gene of wheat chloroplast. The gene, 243 bp in length, is located in a 7.5-kb BamHI fragment generated from the 14.8-kb PvuII fragment in the large single-copy region of the chloroplast genome. The nucleotide sequence of the gene has been determined. The deduced amino acid (aa) sequence shows 2 aa changes relative to its homologue from wheat and spinach and 1 aa with respect to that found in tobacco chloroplast. The gene is apparently transcribed independently as an 850-nt transcript.