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Literature DB >> 2449348

Commercially available fluorescein-conjugated monoclonal antibody for determining the in vitro activity of antimicrobial agents against Chlamydia trachomatis.

J M Webberley¹, R S Matthews, J M Andrews, R Wise.

Abstract

A simple, reproducible method for determining the antibiotic susceptibility of chlamydial isolates is described. Minimum inhibitory and lethal concentrations (MICs and MLCs) were determined for tetracycline and erythromycin titrated against a recent clinical isolate of Chlamydia trachomatis in McCoy cell cultures. A fluorescent antibody stain was found to be more sensitive than giemsa staining, generally giving two-fold higher values for both MICs and MLCs.

Entities: Chemical Species

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Year: 1987 PMID： 2449348 DOI： 10.1007/bf02014256

Source DB: PubMed Journal: Eur J Clin Microbiol ISSN： 0722-2211 Impact factor: 3.267

7 in total

1. In vitro assays of the efficacy of antimicrobial agents in controlling Chlamydia trachomatis propagation.

Authors: C K Lee; W R Bowie; E R Alexander
Journal: Antimicrob Agents Chemother Date: 1978-03 Impact factor: 5.191

2. Antimicrobial activity of several antibiotics and a sulfonamide against Chlamydia trachomatis organisms in cell culture.

Authors: C C Kuo; S P Wang; J T Grayston
Journal: Antimicrob Agents Chemother Date: 1977-07 Impact factor: 5.191

3. Comparative in vitro activity of RU 28965 against Chlamydia trachomatis.

Authors: R Cevenini; V Sambri; M La Placa
Journal: Eur J Clin Microbiol Date: 1986-10 Impact factor: 3.267

4. Early detection of chlamydial inclusions combining the use of cycloheximide-treated McCoy cells and immunofluorescence staining.

Authors: B J Thomas; R T Evans; G R Hutchinson; D Taylor-Robinson
Journal: J Clin Microbiol Date: 1977-09 Impact factor: 5.948

5. A comparison of the in-vitro activity of antimicrobials against Chlamydia trachomatis examined by Giemsa and a fluorescent antibody stain.

Authors: S J How; D Hobson; C A Hart; E Quayle
Journal: J Antimicrob Chemother Date: 1985-04 Impact factor: 5.790

6. Comparison of the in vitro activities of ofloxacin and tetracycline against Chlamydia trachomatis as assessed by indirect immunofluorescence.

Authors: J M Bailey; C Heppleston; S J Richmond
Journal: Antimicrob Agents Chemother Date: 1984-07 Impact factor: 5.191

Commercially available fluorescein-conjugated monoclonal antibody for determining the in vitro activity of antimicrobial agents against Chlamydia trachomatis.

1. In vitro assays of the efficacy of antimicrobial agents in controlling Chlamydia trachomatis propagation.

2. Antimicrobial activity of several antibiotics and a sulfonamide against Chlamydia trachomatis organisms in cell culture.

3. Comparative in vitro activity of RU 28965 against Chlamydia trachomatis.

4. Early detection of chlamydial inclusions combining the use of cycloheximide-treated McCoy cells and immunofluorescence staining.

5. A comparison of the in-vitro activity of antimicrobials against Chlamydia trachomatis examined by Giemsa and a fluorescent antibody stain.

6. Comparison of the in vitro activities of ofloxacin and tetracycline against Chlamydia trachomatis as assessed by indirect immunofluorescence.

7. The antimicrobial susceptibility of Chlamydia trachomatis in cell culture.

1. In vitro activity of BAY 12-8039, a new fluoroquinolone.

2. Antimicrobial susceptibility testing of Chlamydia trachomatis using a reverse transcriptase PCR-based method.

3. In vitro activities of peptide deformylase inhibitors against gram-positive pathogens.

4. In vitro activity of lomefloxacin, a new quinolone antimicrobial agent, in comparison with those of other agents.

5. In vitro activity of PD 127,391, an enhanced-spectrum quinolone.

6. In vitro activities of two glycylcyclines.

7. Evaluating the Antibiotic Susceptibility of Chlamydia - New Approaches for in Vitro Assays.