Secretion of photosynthetic products by carrot tissue cultures.

Green carrot callus cultures when exposed to (14)CO2 in a liquid medium showed ligh-dependent (14)C-incorporation into sucrose, glutamine and malic acid. About 5% of total (14)C fixed in a 3 h period appeared in these products in the bathing medium; this was not due to tissue damage. Kinetic studies showed that the release occurred from a metabolic and not a storage compartment. The effects of DCMU, temperature and fluoroacetate demonstrated that release from this compartment was under respiratory and not photosynthetic control.