Literature DB >> 24491566

Efficient generation of genome-modified mice via offset-nicking by CRISPR/Cas system.

Wataru Fujii1, Asuka Onuma2, Koji Sugiura2, Kunihiko Naito3.   

Abstract

The mammalian zygote-mediated CRISPR/Cas system can efficiently generate targeted genome-modified animals. However, this system is limited by the risk of off-target mutations. Here we show that offset-nicking by Cas9 nickase and paired gRNAs allows us to generate region deleted mice and targeted knock-in mice without off-target mutations.
Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.

Entities:  

Keywords:  CRISPR/Cas9; Genome-modified animals; Nickase; Off-target

Mesh：

Substances：

Year:  2014        PMID： 24491566     DOI： 10.1016/j.bbrc.2014.01.141

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  30 in total

Review 1.  Gene editing and its application for hematological diseases.

Authors:  Mark J Osborn; Joseph J Belanto; Jakub Tolar; Daniel F Voytas
Journal:  Int J Hematol       Date:  2016-05-27       Impact factor: 2.490

2.  Mouse Genome Editing Using the CRISPR/Cas System.

Authors:  Donald W Harms; Rolen M Quadros; Davide Seruggia; Masato Ohtsuka; Gou Takahashi; Lluis Montoliu; Channabasavaiah B Gurumurthy
Journal:  Curr Protoc Hum Genet       Date:  2014-10-01

3.  Somatic mosaicism and allele complexity induced by CRISPR/Cas9 RNA injections in mouse zygotes.

Authors:  Shuo-Ting Yen; Min Zhang; Jian Min Deng; Shireen J Usman; Chad N Smith; Jan Parker-Thornburg; Paul G Swinton; James F Martin; Richard R Behringer
Journal:  Dev Biol       Date:  2014-06-28       Impact factor: 3.582

Review 4.  The new CRISPR-Cas system: RNA-guided genome engineering to efficiently produce any desired genetic alteration in animals.

Authors:  Davide Seruggia; Lluis Montoliu
Journal:  Transgenic Res       Date:  2014-08-06       Impact factor: 2.788

5.  Herpes simplex virus-1 evasion of CD8+ T cell accumulation contributes to viral encephalitis.

Authors:  Naoto Koyanagi; Takahiko Imai; Keiko Shindo; Ayuko Sato; Wataru Fujii; Takeshi Ichinohe; Naoki Takemura; Shigeru Kakuta; Satoshi Uematsu; Hiroshi Kiyono; Yuhei Maruzuru; Jun Arii; Akihisa Kato; Yasushi Kawaguchi
Journal:  J Clin Invest       Date:  2017-09-11       Impact factor: 14.808

6.  Deletion of a target gene in Indica rice via CRISPR/Cas9.

Authors:  Ying Wang; Lizhao Geng; Menglong Yuan; Juan Wei; Chen Jin; Min Li; Kun Yu; Ya Zhang; Huaibing Jin; Eric Wang; Zhijian Chai; Xiangdong Fu; Xianggan Li
Journal:  Plant Cell Rep       Date:  2017-06-05       Impact factor: 4.570

7.  Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes.

Authors:  Yoshiko Nakagawa; Tetsushi Sakuma; Takuya Sakamoto; Masaki Ohmuraya; Naomi Nakagata; Takashi Yamamoto
Journal:  BMC Biotechnol       Date:  2015-05-22       Impact factor: 2.563

8.  Application of CRISPR/Cas9 for biomedical discoveries.

Authors:  Sean M Riordan; Daniel P Heruth; Li Q Zhang; Shui Qing Ye
Journal:  Cell Biosci       Date:  2015-06-21       Impact factor: 7.133

9.  Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system.

Authors:  Tetsushi Sakuma; Ayami Nishikawa; Satoshi Kume; Kazuaki Chayama; Takashi Yamamoto
Journal:  Sci Rep       Date:  2014-06-23       Impact factor: 4.379

10.  One-step generation of phenotype-expressing triple-knockout mice with heritable mutated alleles by the CRISPR/Cas9 system.

Authors:  Wataru Fujii; Asuka Onuma; Koji Sugiura; Kunihiko Naito
Journal:  J Reprod Dev       Date:  2014-05-04       Impact factor: 2.214
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