Literature DB >> 24487100

Evaluation of an automated SPF10-LiPA25 assay for detection and typing of human papillomavirus in archival samples.

Alyssa M Cornall1, Wim H Quint2, Suzanne M Garland3, Sepehr N Tabrizi4.   

Abstract

An automated blotter was evaluated for detection of the HPV SPF10-LiPA25 RHA for HPV genotyping with 63 FFPE tissue and 45 WHO proficiency panel samples. The results showed that 10-20% more types were detected and bands were of significantly stronger intensity (p<0.0001) compared to manual processing. Therefore it was concluded that automated detection of HPV genotypes using the HPV SPF10-LiPA25 is more sensitive for low copy number and mixed-type HPV samples.
Copyright © 2014 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Automation; FFPE; Genotyping; HPV; SPF10-LiPA

Mesh：

Year:  2014        PMID： 24487100     DOI： 10.1016/j.jviromet.2014.01.012

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  3 in total

1.  Human Papillomavirus Genotype Detection in Oral Gargle Samples Among Men With Newly Diagnosed Oropharyngeal Squamous Cell Carcinoma.

Authors:  Laura Martin-Gomez; Anna R Giuliano; William J Fulp; Jimmy Caudell; Michelle Echevarria; Bradley Sirak; Martha Abrahamsen; Kimberly A Isaacs-Soriano; Juan C Hernandez-Prera; Bruce M Wenig; Kathryn Vorwald; Caitlin P McMullen; J Trad Wadsworth; Robbert J Slebos; Christine H Chung
Journal:  JAMA Otolaryngol Head Neck Surg       Date:  2019-05-01       Impact factor: 6.223

2.  Improving oral human papillomavirus detection using toothbrush sampling in HIV-positive men who have sex with men.

Authors:  Jason J Ong; Tim Read; Marcus Chen; Sandra Walker; Matthew Law; Catriona Bradshaw; Suzanne M Garland; Sepehr N Tabrizi; Alyssa Cornall; Andrew Grulich; Jane Hocking; Christopher K Fairley
Journal:  J Clin Microbiol       Date:  2014-04-09       Impact factor: 5.948

3.  Oral gargle-tumor biopsy human papillomavirus (HPV) agreement and associated factors among oropharyngeal squamous cell carcinoma (OPSCC) cases.

Authors:  Laura Martin-Gomez; William J Fulp; Michael J Schell; Bradley Sirak; Martha Abrahamsen; Kimberly A Isaacs-Soriano; Attila Lorincz; Bruce Wenig; Christine H Chung; Jimmy J Caudell; Anna R Giuliano
Journal:  Oral Oncol       Date:  2019-04-03       Impact factor: 5.337
  3 in total

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