| Literature DB >> 24486621 |
Maï Panchal1, Mathieu Gaudin2, Adina N Lazar3, Elisa Salvati4, Isabelle Rivals5, Sophie Ayciriex6, Luce Dauphinot7, Delphine Dargère6, Nicolas Auzeil6, Massimo Masserini4, Olivier Laprévote8, Charles Duyckaerts9.
Abstract
The senile plaque is a hallmark lesion of Alzheimer disease (AD). We compared, without a priori, the lipidome of the senile plaques and of the adjacent plaque-free neuropil. The analysis by liquid chromatography coupled with electrospray ionization mass spectrometry revealed that laser microdissected senile plaques were enriched in saturated ceramides Cer(d18:1/18:0) and Cer(d18:1/20:0) by 33 and 78% respectively with respect to the surrounding neuropil. This accumulation of ceramides was not explained by their affinity for Aβ deposits: no interaction between ceramide-liposomes and Aβ fibrils was observed in vitro by surface plasmon resonance and fluorescent ceramide-liposomes showed no affinity for the senile plaques in AD brain tissue. Accumulation of ceramides could be, at least partially, the result of a local production by acid and neutral sphingomyelinases that we found to be present in the corona of the senile plaques.Entities:
Keywords: Alzheimer disease; Aβ peptide; Ceramides; Laser microdissection; Lipidomics; Lipids; Mass spectrometry; Senile plaques; Sphingomyelinase
Mesh：
Substances：
Year: 2014 PMID： 24486621 DOI： 10.1016/j.nbd.2014.01.010
Source DB: PubMed Journal: Neurobiol Dis ISSN： 0969-9961 Impact factor: 5.996