Roger D Madison1, Grant A Robinson2, Christian Krarup3, Mihai Moldovan3, Qiang Li4, Wilkie A Wilson5. 1. Surgery Department, Duke University Medical Center, Durham, NC 27710, United States; Research Service of the Veterans Affairs Medical Center, Durham, NC 27705, United States. Electronic address: madis001@mc.duke.edu. 2. Surgery Department, Duke University Medical Center, Durham, NC 27710, United States. Electronic address: robin038@mc.duke.edu. 3. Clinical Neurophysiology Department, Neuroscience Center, Rigshospitalet, Copenhagen, Denmark. 4. Psychiatry Department, Duke University Medical Center, Durham, NC 27710, United States; Research Service of the Veterans Affairs Medical Center, Durham, NC 27705, United States. 5. Social Sciences Research Institute, Duke University Medical Center, Durham, NC 27710, United States; Research Service of the Veterans Affairs Medical Center, Durham, NC 27705, United States.
Abstract
BACKGROUND: Given the movement of molecules within tissue that occurs naturally by endogenous electric fields, we examined the possibility of using a low-voltage DC field to move charged substances in rodent peripheral nerve in vitro. NEW METHOD: Labeled sugar- and protein-based markers were applied to a rodent peroneal nerve and then a 5-10 V/cm field was used to move the molecules within the extra- and intraneural compartments. Physiological and anatomical nerve properties were also assessed using the same stimulation in vivo. RESULTS: We demonstrate in vitro that charged and labeled compounds are capable of moving in a DC field along a nerve, and that the same field applied in vivo changes the excitability of the nerve, but without damage. CONCLUSIONS: The results suggest that low-voltage electrophoresis could be used to move charged molecules, perhaps therapeutically, safely along peripheral nerves.
BACKGROUND: Given the movement of molecules within tissue that occurs naturally by endogenous electric fields, we examined the possibility of using a low-voltage DC field to move charged substances in rodent peripheral nerve in vitro. NEW METHOD: Labeled sugar- and protein-based markers were applied to a rodent peroneal nerve and then a 5-10 V/cm field was used to move the molecules within the extra- and intraneural compartments. Physiological and anatomical nerve properties were also assessed using the same stimulation in vivo. RESULTS: We demonstrate in vitro that charged and labeled compounds are capable of moving in a DC field along a nerve, and that the same field applied in vivo changes the excitability of the nerve, but without damage. CONCLUSIONS: The results suggest that low-voltage electrophoresis could be used to move charged molecules, perhaps therapeutically, safely along peripheral nerves.
Authors: Mihai Moldovan; Susana Alvarez; Volodymyr Pinchenko; Dennis Klein; Finn Cilius Nielsen; John N Wood; Rudolf Martini; Christian Krarup Journal: Brain Date: 2010-12-17 Impact factor: 13.501
Authors: Brandon M Wild; Renée Morris; Mihai Moldovan; Christian Krarup; Arun V Krishnan; Ria Arnold Journal: J Vis Exp Date: 2018-02-06 Impact factor: 1.355