Literature DB >> 24485745

Bio-imaging of hydroxyl radicals in plant cells using the fluorescent molecular probe rhodamine B hydrazide, without any pretreatment.

Mamiko Asano1, Mitsunobu Doi2, Kimiye Baba2, Masahiko Taniguchi2, Makio Shibano2, Satoshi Tanaka2, Minoru Sakaguchi2, Masanori Takaoka2, Masahiko Hirata2, Rika Yanagihara2, Ryosuke Nakahara3, Yuko Hayashi2, Takako Yamaguchi2, Hitoshi Matsumura2, Yoshikazu Fujita2.   

Abstract

Rhodamine B hydrazide can be used to detect hydroxyl radicals in plant cells. RBH was easily inserted into plant cells without any pretreatment, and specifically reacted with intracellular hydroxyl radicals produced by antimycin A. RBH will be a powerful tool for detecting hydroxyl radicals in plant cells.
Copyright © 2013 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Hydroxyl radical; Nicotiana tabacum BY-2; Onion epidermal cell; Reactive oxygen species; Rhodamine B hydrazide

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Substances：

Year:  2014        PMID： 24485745     DOI： 10.1016/j.jbiosc.2013.12.015

Source DB:  PubMed          Journal:  J Biosci Bioeng        ISSN： 1347-4421            Impact factor:   2.894


  2 in total

1.  In vivo imaging of reactive oxygen species in mouse brain by using [3H]hydromethidine as a potential radical trapping radiotracer.

Authors:  Kohji Abe; Nozomi Takai; Kazumi Fukumoto; Natsumi Imamoto; Misato Tonomura; Miwa Ito; Naoki Kanegawa; Katsunori Sakai; Kenji Morimoto; Kenichiro Todoroki; Osamu Inoue
Journal:  J Cereb Blood Flow Metab       Date:  2014-09-17       Impact factor: 6.200

2.  Imaging of reactive oxygen species in focal ischemic mouse brain using a radical trapping tracer [(3)H]hydromethidine.

Authors:  Kohji Abe; Misato Tonomura; Miwa Ito; Nozomi Takai; Natsumi Imamoto; Takemi Rokugawa; Sotaro Momosaki; Kazumi Fukumoto; Kenji Morimoto; Osamu Inoue
Journal:  EJNMMI Res       Date:  2015-06-26       Impact factor: 3.138
  2 in total

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