Mao Qian1, Li Yi2, Li Song-Lin1, Yang Jie3, Zhang Ping-Hu4, Wang Qiang5. 1. Department of Pharmaceutical Analysis and Metabolomics, Jiangsu Branch of China Academy of Chinese Medical Science, Nanjing 210009, China. 2. Nanjing Normal University Center for Analysis and Testing, Nanjing Normal University, Nanjing 210009, China. 3. State Laboratory of Modern Chinese Medicines, China Pharmaceutical University, Nanjing 210009, China. 4. Jiangsu Center for New Drug Screening & National New Drug Screening Laboratory, China Pharmaceutical University, Nanjing 210009, China. Electronic address: zhangpinghu@163.com. 5. State Laboratory of Modern Chinese Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address: qwang49@126.com.
Abstract
AIM: To evaluate the chemical profiles and cytotoxic effects among the total saponin fraction (TSF), 25% ethanol fraction (25EF), 50% ethanol fraction (50EF), and 85% ethanol fraction (85EF) prepared by macroporous resin from the leaves of Panax notoginseng. METHOD: The simultaneous determination of thirteen main saponins, as well as the chemical profiles of saponin fractions of different polarity, was made by HPLC-DAD and LC-ESI-MS(n) analysis. The cytotoxic effects were determined against KP4 cells (human pancreatic cancer), NCI-H727 cells (human lung cancer), HepG2 cells (human hepatocellular cancer), and SGC-7901 cells (human gastric adenocarcinoma). RESULTS: Chemical analysis indicated that 85EF possessed the most abundant cytotoxic protopanaxadiol saponins, including the marker saponins F2, 20(R)-Rg3, 20(S)-Rg3, and Rh2. The MTT assay showed that 85EF also had the strongest cytotoxic effects among the four fractions. 25EF showed no anti-proliferative effects, while 50EF and TSF exhibited weak anti-proliferative activity. CONCLUSION: From the aspect of comprehensive utilization of resources, 85EF, enriched with low polarity PPD group saponins, is a new alternative source of anticancer saponins, and a promising botanical preparation for further anticancer studies.
AIM: To evaluate the chemical profiles and cytotoxic effects among the total saponin fraction (TSF), 25% ethanol fraction (25EF), 50% ethanol fraction (50EF), and 85% ethanol fraction (85EF) prepared by macroporous resin from the leaves of Panax notoginseng. METHOD: The simultaneous determination of thirteen main saponins, as well as the chemical profiles of saponin fractions of different polarity, was made by HPLC-DAD and LC-ESI-MS(n) analysis. The cytotoxic effects were determined against KP4 cells (humanpancreatic cancer), NCI-H727 cells (humanlung cancer), HepG2 cells (humanhepatocellular cancer), and SGC-7901 cells (humangastric adenocarcinoma). RESULTS: Chemical analysis indicated that 85EF possessed the most abundant cytotoxic protopanaxadiolsaponins, including the marker saponins F2, 20(R)-Rg3, 20(S)-Rg3, and Rh2. The MTT assay showed that 85EF also had the strongest cytotoxic effects among the four fractions. 25EF showed no anti-proliferative effects, while 50EF and TSF exhibited weak anti-proliferative activity. CONCLUSION: From the aspect of comprehensive utilization of resources, 85EF, enriched with low polarity PPD group saponins, is a new alternative source of anticancer saponins, and a promising botanical preparation for further anticancer studies.
Authors: Yawen Zeng; Juan Du; Xiaoying Pu; Jiazhen Yang; Tao Yang; Shuming Yang; Xiaomeng Yang Journal: Biomed Res Int Date: 2015-11-01 Impact factor: 3.411