Literature DB >> 24482706

Expression of E6, p53 and p21 proteins and physical state of HPV16 in cervical cytologies with and without low grade lesions.

Diana K Jiménez Tagle1, Daniel Hernández Sotelo2, Berenice Illades-Aguiar2, Marco A Leyva-Vazquez2, Eugenia Flores Alfaro3, Yaneth Castro Coronel1, Oscar Del Moral Hernández2, Luz Del Carmen Alarcón Romero1.   

Abstract

The aim of this study was to determine the correlation between expression of HPV16 E6, p53 and p21 proteins and the physical state of HPV16 in cervical cytologies without squamous intraepithelial lesions (Non-SIL) and with low grade squamous intraepithelial lesions (LSIL), both with HPV16 infection. 101 liquid-based cytological samples were analyzed. 50 samples were without squamous intraepithelial lesions (Non-IL) and 51 samples of low grade squamous intraepithelial lesions (LSIL), both with HPV16 infection. HPV16 infection was determined by PCR-RFLP, and the physical state of HPV16 by in situ hybridization with tyramide-amplification. The expression of E6, p53 and p21 proteins was evaluated by immunocytochemistry. The expression of HPV16 E6 protein was significantly higher in LSIL that in Non-SIL samples (p=0.006). We found a significant correlation between E6 expression and the physical state of HPV16 in Non-SIL (p=0.049). Our results suggest that high expression of E6 in LSIL is an early event of cervical carcinogenesis and perhaps can be used as an early marker.

Entities:  

Keywords:  E6; HPV16; LSIL; cervical cancer; immunocytochemistry; in situ hybridization; liquid-based cytological; p21; p53; physical state

Year:  2014        PMID： 24482706      PMCID： PMC3902258     

Source DB:  PubMed          Journal:  Int J Clin Exp Med        ISSN： 1940-5901


  43 in total

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Journal:  Oncol Lett       Date:  2013-05-20       Impact factor: 2.967
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  5 in total

1.  Retrospective analysis of HPV 16/18-related disease burden using archival clinical samples.

Authors:  Naureen Ehsan Ilahi; Shoaib Naiyar Hashmi; Sobia Anwar; Sheeba Murad
Journal:  J Cancer Res Clin Oncol       Date:  2016-08-30       Impact factor: 4.553

2.  Delineation of the HPV11E6 and HPV18E6 Pathways in Initiating Cellular Transformation.

Authors:  Lamech M Mwapagha; Nicki Tiffin; M Iqbal Parker
Journal:  Front Oncol       Date:  2017-11-01       Impact factor: 6.244

Review 3.  The Association and Significance of p53 in Gynecologic Cancers: The Potential of Targeted Therapy.

Authors:  Mitsuhiro Nakamura; Takeshi Obata; Takiko Daikoku; Hiroshi Fujiwara
Journal:  Int J Mol Sci       Date:  2019-11-04       Impact factor: 5.923

4.  TOP2A/MCM2, p16INK4a, and cyclin E1 expression in liquid-based cytology: a biomarkers panel for progression risk of cervical premalignant lesions.

Authors:  Oscar Del Moral-Hernández; Daniel Hernández-Sotelo; Luz Del Carmen Alarcón-Romero; Miguel Angel Mendoza-Catalán; Eugenia Flores-Alfaro; Yaneth Castro-Coronel; Julio Ortiz-Ortiz; Marco Antonio Leyva-Vázquez; Carlos Ortuño-Pineda; Wendy Castro-Mora; Berenice Illades-Aguiar
Journal:  BMC Cancer       Date:  2021-01-07       Impact factor: 4.430

Review 5.  Biomarkers of HIV-associated Cancer.

Authors:  Brian Thabile Flepisi; Patrick Bouic; Gerhard Sissolak; Bernd Rosenkranz
Journal:  Biomark Cancer       Date:  2014-07-03
  5 in total

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