Literature DB >> 24482176

Radioactive in situ hybridization to detect gene expression in skeletal tissue sections.

Kyu Sang Joeng1, Jenna Regan2, Fanxin Long2.   

Abstract

In situ hybridization (ISH) using RNA probes is a valuable technique to characterize gene expression patterns in animal tissues. It provides valuable spatial information about gene expression. Compared to the nonradioactive alternatives,(35)S radioactive ISH generally provides higher sensitivity. Here, we describe the procedure for(35)S ISH on paraffin sections from the skeletal tissues of mouse embryos.

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Year:  2014        PMID: 24482176     DOI: 10.1007/978-1-62703-989-5_16

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  2 in total

1.  Chondrocyte-Specific RUNX2 Overexpression Accelerates Post-traumatic Osteoarthritis Progression in Adult Mice.

Authors:  Sarah E Catheline; Donna Hoak; Martin Chang; John P Ketz; Matthew J Hilton; Michael J Zuscik; Jennifer H Jonason
Journal:  J Bone Miner Res       Date:  2019-06-12       Impact factor: 6.741

2.  Preparation of Adult Mouse Skeletal Tissue Sections for RNA In Situ Hybridization.

Authors:  Charles de Charleroy; Abdul Haseeb; Véronique Lefebvre
Journal:  Methods Mol Biol       Date:  2021
  2 in total

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