Literature DB >> 24481617

Glucosyltransferase activity in a water extract of maize pollen.

R L Larson1, C M Lonergan.   

Abstract

Glucosyltransferase activity was extracted from maize pollen in distilled water. The enzymatic reaction required UDP-glucose, mercaptoethanol and Ca(++), and had a pH optimum at 8.2. Either kampferol or quercetin served as a substrate. Michaelis-Menten constants obtained were 0.6×10(-4) M for quercetin and 0.74×10(-3)M for UDP-glucose. Ammonium-sulfate precipitation of the enzyme gave a 4fold purification.

Entities:  

Year:  1972        PMID： 24481617     DOI： 10.1007/BF00386709

Source DB:  PubMed          Journal:  Planta        ISSN： 0032-0935            Impact factor:   4.116


  4 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Phosphatases of ragweed pollen.

Authors:  G R NAKAMURA; E L BECKER
Journal:  Arch Biochem Biophys       Date:  1951-08       Impact factor: 4.013

3.  The occurrence of quercetin in the pollen of Zea mays.

Authors:  C T REDEMANN; S H WITTWER
Journal:  Arch Biochem       Date:  1950-02

4.  Magnesol thin-layer chromatography of flavonoid compounds.

Authors:  R L Larson
Journal:  J Chromatogr       Date:  1969-08-19
  4 in total
  2 in total

1.  Genetic control of UDPglucose:flavonol 3-O-glucosyltransferase in the endosperm of maize.

Authors:  H K Dooner; O E Nelson
Journal:  Biochem Genet       Date:  1977-06       Impact factor: 1.890

2.  Identification, properties and genetic control of UDP-glucose: isovitexin 7-O-glucosyltransferase isolated from petals of Melandrium album.

Authors:  J van Brederode; G van Nigtevecht
Journal:  Mol Gen Genet       Date:  1973-05-09
  2 in total

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