Literature DB >> 2448151

BrdU-Hoechst flow cytometry: a unique tool for quantitative cell cycle analysis.

P S Rabinovitch1, M Kubbies, Y C Chen, D Schindler, H Hoehn.   

Abstract

Unlike other techniques, flow cytometric analysis of BrdU-quenched 33258 Hoechst fluorescence may be used to measure cell activation and the G1, S, and G2/M compartment distributions in each of three successive cell cycles after growth stimulation of human peripheral blood lymphocytes. Cell cycle kinetic curves can be constructed from the BrdU-Hoechst flow data which allow the simultaneous assessment of growth fraction, lag-time, compartment exit rate, compartment duration, and compartment arrest. Applications of this new versatile technique include the evaluation of drug and growth factor effects, cell aging, and diagnosis in medicine and immunology.

Entities:  

Mesh：

Substances：

Year:  1988        PMID： 2448151     DOI： 10.1016/0014-4827(88)90302-3

Source DB:  PubMed          Journal:  Exp Cell Res        ISSN： 0014-4827            Impact factor:   3.905


  20 in total

1.  Potential mechanisms of human natural killer cell expansion in vivo during low-dose IL-2 therapy.

Authors:  T A Fehniger; E M Bluman; M M Porter; E Mrózek; M A Cooper; J B VanDeusen; S R Frankel; W Stock; M A Caligiuri
Journal:  J Clin Invest       Date:  2000-07       Impact factor: 14.808

2.  The potential of flow cytometric analysis for the characterization of hybridoma cells in suspension cultures.

Authors:  J M Coco-Martin; J W Oberink; T A van der Velden-de Groot; E C Beuvery
Journal:  Cytotechnology       Date:  1992       Impact factor: 2.058

3.  A rapid survival assay to measure drug-induced cytotoxicity and cell cycle effects.

Authors:  Chandni Valiathan; Jose L McFaline; Leona D Samson
Journal:  DNA Repair (Amst)       Date:  2011-11-30

4.  The murine long-term multi-lineage renewal marrow stem cell is a cycling cell.

Authors:  L R Goldberg; M S Dooner; K W Johnson; E F Papa; M G Pereira; M Del Tatto; D M Adler; J M Aliotta; P J Quesenberry
Journal:  Leukemia       Date:  2013-08-30       Impact factor: 11.528

5.  Cell kinetic disturbances induced by treatment of human diploid fibroblasts with 5-azacytidine indicate a major role for DNA methylation in the regulation of the chromosome cycle.

Authors:  M Poot; J Koehler; P S Rabinovitch; H Hoehn; J H Priest
Journal:  Hum Genet       Date:  1990-02       Impact factor: 4.132

6.  Serum factors can modulate the developmental clock of gamma- to beta-globin gene switching in somatic cell hybrids.

Authors:  G Zitnik; Q Li; G Stamatoyannopoulos; T Papayannopoulou
Journal:  Mol Cell Biol       Date:  1993-08       Impact factor: 4.272

7.  The Ca2(+)-binding glycoprotein SPARC modulates cell cycle progression in bovine aortic endothelial cells.

Authors:  S E Funk; E H Sage
Journal:  Proc Natl Acad Sci U S A       Date:  1991-04-01       Impact factor: 11.205

8.  New mutations in the ATM gene and clinical data of 25 AT patients.

Authors:  Ilja Demuth; Véronique Dutrannoy; Wilson Marques; Heidemarie Neitzel; Detlev Schindler; Petja S Dimova; Krystyna H Chrzanowska; Veneta Bojinova; Hanna Gregorek; Luitgard M Graul-Neumann; Arpad von Moers; Ilka Schulze; Marion Nicke; Elcin Bora; Tufan Cankaya; Éva Oláh; Csongor Kiss; Beáta Bessenyei; Katalin Szakszon; Ursula Gruber-Sedlmayr; Peter Michael Kroisel; Sigrun Sodia; Timm O Goecke; Thilo Dörk; Martin Digweed; Karl Sperling; Joaquim de Sá; Charles Marques Lourenco; Raymonda Varon
Journal:  Neurogenetics       Date:  2011-10-02       Impact factor: 2.660

9.  G2 phase cell cycle disturbance as a manifestation of genetic cell damage.

Authors:  H Seyschab; Y Sun; R Friedl; D Schindler; H Hoehn
Journal:  Hum Genet       Date:  1993-08       Impact factor: 4.132

10.  Simultaneous measurement, using flow cytometry, of radiosensitivity and defective mitogen response in ataxia telangiectasia and related syndromes.

Authors:  H Seyschab; D Schindler; R Friedl; G Barbi; E Boltshauser; J P Fryns; F Hanefeld; R Korinthenberg; I Krägeloh-Mann; J M Scheres
Journal:  Eur J Pediatr       Date:  1992-10       Impact factor: 3.183
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.