| Literature DB >> 24481275 |
Hang-jun Wu1, Yi-jun Liu2, Hui-quan Li2, Cong Chen2, Ying Dou2, Hui-fang Lou2, Margaret S Ho3, Xiao-ming Li2, Zhihua Gao2, Shumin Duan2.
Abstract
Microglial cells have important roles in maintaining brain homeostasis, and they are implicated in multiple brain diseases. There is currently interest in investigating microglial migration that results in cell accumulation at focal sites of injury. Here we describe a protocol for rapidly triggering and monitoring microglial migration by using a micropipette assay. This protocol is an adaptation of the axon turning assay using microglial cells. Chemoattractants released from the micropipette tip produce a chemotactic gradient that induces robust microglial migration. In combination with microscopic imaging, this assay allows simultaneous recording of cell movement and subcellular compartment trafficking, along with quantitative analysis. The actual handling time for the assay takes ∼2-3 h in total. The protocol is simple, inexpensive and convenient to set up, and it can be adopted to examine cell migration in multiple cell types, including cancer cells with a wide range of chemical signals.Entities:
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Year: 2014 PMID： 24481275 DOI： 10.1038/nprot.2014.015
Source DB: PubMed Journal: Nat Protoc ISSN： 1750-2799 Impact factor: 13.491