Qile Gao1, Qingbiao Du2, Hongqi Zhang3, Chaofeng Guo1, Shijin Lu1, Ang Deng1, Mingxing Tang1, Shaohua Liu1, Yuxiang Wang1, Jia Huang1, Qiang Guo1. 1. Department of Spine Surgery, Xiangya Hospital, Central South University, Hunan, China. 2. Clinical Laboratory, Secondary Affiliated Hospital, Fujian Medical University, Fujian, China. 3. Department of Spine Surgery, Xiangya Hospital, Central South University, Hunan, China. Electronic address: zhq9996@aliyun.com.
Abstract
BACKGROUND AND AIMS: Monocyte chemotactic protein-1 (MCP-1) gene polymorphisms play important roles in regulating immunological reactions and may be associated with pulmonary tuberculosis. However, the relationship between the MCP-1 -2518 gene polymorphism and susceptibility to spinal tuberculosis remains unknown. We undertook this study to investigate the relationships between MCP-1 promoter 2518 genotype frequency and allele polymorphisms and susceptibility to spinal tuberculosis in a Chinese Han population. METHODS: Patients with spinal tuberculosis and healthy volunteers were enrolled between December 2004 and December 2010. MCP-1 -2518 polymorphisms in both groups were detected using polymerase chain reaction and DNA sequencing. MCP-1 genotype was analyzed in all patients. Differences in genotype frequencies between groups were compared using χ(2) tests. RESULTS: A total of 208 patients with spinal tuberculosis and 210 healthy volunteers were included. The distribution frequencies of MCP-1 -2518 GG, GA and AA genotypes were 36.1, 50.9 and 13.0%, respectively, in the case group and 25.2, 53.8 and 21.0%, respectively, in the control group (p <0.05). MCP-1 -2518 GG genotype was significantly associated with the onset of spinal tuberculosis (OR = 2.306, 95% CI = 1.273-4.178). The G and A allele frequencies were 61.5% and 38.5%, respectively, in the case group, and 52.1% and 47.9% in the control group (p <0.05), the allele "G" of MCP-1 -2518 showed an association with an increased risk for spinal tuberculosis: OR = 1.777, 95% CI = 1.053-2999, p = 0.03 in the dominant model; OR = 1.67, 95% CI = 1.097-2.544, p = 0.016 in the recessive model. CONCLUSIONS: The MCP-1 -2518 GG genotype and presence of the G allele may be associated with susceptibility to spinal tuberculosis in the Chinese Han population.
BACKGROUND AND AIMS: Monocyte chemotactic protein-1 (MCP-1) gene polymorphisms play important roles in regulating immunological reactions and may be associated with pulmonary tuberculosis. However, the relationship between the MCP-1 -2518 gene polymorphism and susceptibility to spinal tuberculosis remains unknown. We undertook this study to investigate the relationships between MCP-1 promoter 2518 genotype frequency and allele polymorphisms and susceptibility to spinal tuberculosis in a Chinese Han population. METHODS:Patients with spinal tuberculosis and healthy volunteers were enrolled between December 2004 and December 2010. MCP-1 -2518 polymorphisms in both groups were detected using polymerase chain reaction and DNA sequencing. MCP-1 genotype was analyzed in all patients. Differences in genotype frequencies between groups were compared using χ(2) tests. RESULTS: A total of 208 patients with spinal tuberculosis and 210 healthy volunteers were included. The distribution frequencies of MCP-1 -2518 GG, GA and AA genotypes were 36.1, 50.9 and 13.0%, respectively, in the case group and 25.2, 53.8 and 21.0%, respectively, in the control group (p <0.05). MCP-1 -2518 GG genotype was significantly associated with the onset of spinal tuberculosis (OR = 2.306, 95% CI = 1.273-4.178). The G and A allele frequencies were 61.5% and 38.5%, respectively, in the case group, and 52.1% and 47.9% in the control group (p <0.05), the allele "G" of MCP-1 -2518 showed an association with an increased risk for spinal tuberculosis: OR = 1.777, 95% CI = 1.053-2999, p = 0.03 in the dominant model; OR = 1.67, 95% CI = 1.097-2.544, p = 0.016 in the recessive model. CONCLUSIONS: The MCP-1 -2518 GG genotype and presence of the G allele may be associated with susceptibility to spinal tuberculosis in the Chinese Han population.
Authors: Bart N Green; Claire D Johnson; Scott Haldeman; Erin Griffith; Michael B Clay; Edward J Kane; Juan M Castellote; Shanmuganathan Rajasekaran; Matthew Smuck; Eric L Hurwitz; Kristi Randhawa; Hainan Yu; Margareta Nordin Journal: PLoS One Date: 2018-06-01 Impact factor: 3.240