Literature DB >> 24478418

Simultaneous detection of five enteric viruses associated with gastroenteritis by use of a PCR assay: a single real-time multiplex reaction and its clinical application.

Yixiang Jiang1, Lin Fang, Xiaolu Shi, Hailong Zhang, Yinghui Li, Yiman Lin, Yaqun Qiu, Qingliang Chen, Hui Li, Li Zhou, Qinghua Hu.   

Abstract

We developed a highly sensitive reverse transcription and multiplex real-time PCR (rtPCR) assay that can identify five viruses, including six genogroups, in a single reaction: norovirus genogroups I and II; sapovirus genogroups I, II, IV, and V; human rotavirus A; adenovirus serotypes 40 and 41; and human astrovirus. In comparison to monoplex rtPCR assays, the sensitivities and specificities of the multiplex rtPCR ranged from 75% to 100% and from 99% to 100%, respectively, evaluated on 812 clinical stool specimens.

Entities:  

Mesh：

Year:  2014        PMID： 24478418      PMCID： PMC3993471          DOI： 10.1128/JCM.00245-14

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  27 in total

1.  Viral acute gastroenteritis: clinical and epidemiological features of co-infected patients.

Authors:  Carla Elizabeth de Oliveira Ferreira; Sonia Mara Raboni; Luciane Aparecida Pereira; Meri Bordignon Nogueira; Luine Rosele Renaud Vidal; Sérgio Monteiro Almeida
Journal:  Braz J Infect Dis       Date:  2012 May-Jun       Impact factor: 1.949

2.  Clinical and molecular epidemiology of norovirus infection in adults with acute gastroenteritis in Ji'nan, China.

Authors:  Lintao Sai; Gang Wang; Lihua Shao; Haihong Liu; Yajun Zhang; Chunmei Qu; Lixian Ma
Journal:  Arch Virol       Date:  2013-06-11       Impact factor: 2.574

3.  Evaluation of Luminex xTAG gastrointestinal pathogen analyte-specific reagents for high-throughput, simultaneous detection of bacteria, viruses, and parasites of clinical and public health importance.

Authors:  Jose F Navidad; David J Griswold; M Stephen Gradus; Sanjib Bhattacharyya
Journal:  J Clin Microbiol       Date:  2013-07-12       Impact factor: 5.948

Review 4.  Diagnosis of noncultivatable gastroenteritis viruses, the human caliciviruses.

Authors:  R L Atmar; M K Estes
Journal:  Clin Microbiol Rev       Date:  2001-01       Impact factor: 26.132

Review 5.  Rotavirus infection and the current status of rotavirus vaccines.

Authors:  Shou-Chien Chen; Lia-Beng Tan; Li-Min Huang; Kow-Tong Chen
Journal:  J Formos Med Assoc       Date:  2012-03-29       Impact factor: 3.282

6.  Real-time qualitative PCR for 57 human adenovirus types from multiple specimen sources.

Authors:  Seanne P Buckwalter; Rose Teo; Mark J Espy; Lynne M Sloan; Thomas F Smith; Bobbi S Pritt
Journal:  J Clin Microbiol       Date:  2011-12-14       Impact factor: 5.948

7.  Rotavirus infection and its genetic characterization in non-hospitalized adults with acute gastroenteritis in Shanghai, China.

Authors:  Zhen Shen; Gang Wang; Wanju Zhang; Fangxing Qian; Yang Li; Min Zhang; Shimin Gu; Moying Wang; Feng Lin; Yunwen Hu; Zhenghong Yuan; Jun Zhang
Journal:  Arch Virol       Date:  2013-03-13       Impact factor: 2.574

8.  Multiplex real-time PCR assays for the detection of group C rotavirus, astrovirus, and Subgenus F adenovirus in stool specimens.

Authors:  Kohji Mori; Yukinao Hayashi; Tetsuya Akiba; Miyuki Nagano; Tatsuya Tanaka; Mitsugu Hosaka; Akiko Nakama; Akemi Kai; Kengo Saito; Hiroshi Shirasawa
Journal:  J Virol Methods       Date:  2012-11-14       Impact factor: 2.014

9.  Molecular characterization of a novel astrovirus associated with disease in mink.

Authors:  Christian Mittelholzer; Kjell-Olof Hedlund; Lena Englund; Hans-Henrik Dietz; Lennart Svensson
Journal:  J Gen Virol       Date:  2003-11       Impact factor: 3.891

10.  Gastroenteritis outbreaks caused by Norovirus genotype II.7 in a college in China (Zhuhai, Guangdong) in 2011.

Authors:  Feng Ruan; Ai Jun Tan; Teng Fei Man; Hui Li; Yan Ling Mo; Yi Xiong Lin; Xiao Ling Deng
Journal:  Foodborne Pathog Dis       Date:  2013-07-10       Impact factor: 3.171
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  14 in total

Review 1.  Comprehensive review of human sapoviruses.

Authors:  Tomoichiro Oka; Qiuhong Wang; Kazuhiko Katayama; Linda J Saif
Journal:  Clin Microbiol Rev       Date:  2015-01       Impact factor: 26.132

Review 2.  Human astroviruses.

Authors:  Albert Bosch; Rosa M Pintó; Susana Guix
Journal:  Clin Microbiol Rev       Date:  2014-10       Impact factor: 26.132

Review 3.  Virological and Immunological Outcomes of Coinfections.

Authors:  Naveen Kumar; Shalini Sharma; Sanjay Barua; Bhupendra N Tripathi; Barry T Rouse
Journal:  Clin Microbiol Rev       Date:  2018-07-05       Impact factor: 26.132

4.  Enteropathogens associated with acute diarrhea in children from households with high socioeconomic level in uruguay.

Authors:  Gustavo Varela; Lara Batthyány; María Noel Bianco; Walter Pérez; Lorena Pardo; Gabriela Algorta; Luciana Robino; Ramón Suárez; Armando Navarro; María Catalina Pírez; Felipe Schelotto
Journal:  Int J Microbiol       Date:  2015-03-12

Review 5.  Astrovirus Diagnostics.

Authors:  Philippe Pérot; Marc Lecuit; Marc Eloit
Journal:  Viruses       Date:  2017-01-12       Impact factor: 5.048

Review 6.  Advances in Diagnostic Approaches for Viral Etiologies of Diarrhea: From the Lab to the Field.

Authors:  Yashpal Singh Malik; Atul Kumar Verma; Naveen Kumar; Nadia Touil; Kumaragurubaran Karthik; Ruchi Tiwari; Durlav Prasad Bora; Kuldeep Dhama; Souvik Ghosh; Maged Gomaa Hemida; Ahmed S Abdel-Moneim; Krisztián Bányai; Anastasia N Vlasova; Nobumichi Kobayashi; Raj Kumar Singh
Journal:  Front Microbiol       Date:  2019-09-13       Impact factor: 5.640

7.  Infectious disease symptoms and microbial carriage among French medical students travelling abroad: A prospective study.

Authors:  Thi Loi Dao; Van Thuan Hoang; Tran Duc Anh Ly; Amal Magmoun; Naomie Canard; Tassadit Drali; Florence Fenollar; Laetitia Ninove; Didier Raoult; Philippe Parola; Johan Courjon; Philippe Gautret
Journal:  Travel Med Infect Dis       Date:  2019-12-21       Impact factor: 6.211

8.  Respiratory and gastrointestinal infections at the 2017 Grand Magal de Touba, Senegal: A prospective cohort survey.

Authors:  Van-Thuan Hoang; Ndiaw Goumballa; Thi-Loi Dao; Tran Duc Anh Ly; Laetitia Ninove; Stéphane Ranque; Didier Raoult; Philippe Parola; Cheikh Sokhna; Vincent Pommier de Santi; Philippe Gautret
Journal:  Travel Med Infect Dis       Date:  2019-05-03       Impact factor: 6.211

9.  The 12 Gastrointestinal Pathogens Spectrum of Acute Infectious Diarrhea in a Sentinel Hospital, Shenzhen, China.

Authors:  Hongwei Shen; Jinjin Zhang; Yinghui Li; Sirou Xie; Yixiang Jiang; Yanjie Wu; Yuhui Ye; Hong Yang; Haolian Mo; Chaoman Situ; Qinghua Hu
Journal:  Front Microbiol       Date:  2016-11-30       Impact factor: 5.640

10.  Simultaneous Identification of Ten Bacterial Pathogens Using the Multiplex Ligation Reaction Based on the Probe Melting Curve Analysis.

Authors:  Yixiang Jiang; Lianhua He; Pingfang Wu; Xiaolu Shi; Min Jiang; Yinghui Li; Yiman Lin; Yaqun Qiu; Fang Bai; Yiqun Liao; Qingge Li; RenLi Zhang; Qinghua Hu
Journal:  Sci Rep       Date:  2017-07-19       Impact factor: 4.379
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