Literature DB >> 24477581

Development and molecular characterization of genic molecular markers for grain protein and calcium content in finger millet (Eleusine coracana (L.) Gaertn.).

M Nirgude1, B Kalyana Babu, Y Shambhavi, U M Singh, H D Upadhyaya, Anil Kumar.   

Abstract

Finger millet (Eleusine coracana (L.) Gaertn), holds immense agricultural and economic importance for its high nutraceuticals quality. Finger millets seeds are rich source of calcium and its proteins are good source of essential amino acids. In the present study, we developed 36 EST-SSR primers for the opaque2 modifiers and 20 anchored-SSR primers for calcium transporters and calmodulin for analysis of the genetic diversity of 103 finger millet genotypes for grain protein and calcium contents. Out of the 36 opaque2 modifiers primers, 15 were found polymorphic and were used for the diversity analysis. The highest PIC value was observed with the primer FMO2E33 (0.26), while the lowest was observed FMO2E27 (0.023) with an average value of 0.17. The gene diversity was highest for the primer FMO2E33 (0.33), however it was lowest for FMO2E27 (0.024) at average value of 0.29. The percentage polymorphism shown by opaque2 modifiers primers was 68.23%. The diversity analysis by calcium transporters and calmodulin based anchored SSR loci revealed that the highest PIC was observed with the primer FMCA8 (0.30) and the lowest was observed for FMCA5 (0.023) with an average value of 0.18. The highest gene diversity was observed for primer FMCA8 (0.37), while lowest for FMCA5 (0.024) at an average of 0.21. The opaque2 modifiers specific EST-SSRs could able to differentiate the finger millet genotypes into high, medium and low protein containing genotypes. However, calcium dependent candidate gene based EST-SSRs could broadly differentiate the genotypes based on the calcium content with a few exceptions. A significant negative correlation between calcium and protein content was observed. The present study resulted in identification of highly polymorphic primers (FMO2E30, FMO2E33, FMO2-18 and FMO2-14) based on the parameters such as percentage of polymorphism, PIC values, gene diversity and number of alleles.

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Year:  2014        PMID： 24477581     DOI： 10.1007/s11033-013-2825-7

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  21 in total

1.  Species identification in seven small millet species using polymerase chain reaction-restriction fragment length polymorphism of trnS-psbC gene region.

Authors:  M Parani; K Rajesh; M Lakshmi; L Parducci; A E Szmidt; A Parida
Journal:  Genome       Date:  2001-06       Impact factor: 2.166

2.  Use of SSR, RAPD markers and protein profiles based analysis to differentiate Eleusine coracana genotypes differing in their protein content.

Authors:  Anil Kumar; Netrapal Sharma; Preety Panwar; Arun K Gupta
Journal:  Mol Biol Rep       Date:  2011-12-14       Impact factor: 2.316

3.  A novel feature of microsatellites in plants: a distribution gradient along the direction of transcription.

Authors:  Shigeo Fujimori; Takanori Washio; Kenichi Higo; Yasuhiro Ohtomo; Kazuo Murakami; Kenichi Matsubara; Jun Kawai; Piero Carninci; Yoshihide Hayashizaki; Shoshi Kikuchi; Masaru Tomita
Journal:  FEBS Lett       Date:  2003-11-06       Impact factor: 4.124

4.  Ribosomal DNA variation in finger millet and wild species of Eleusine (Poaceae).

Authors:  K W Hilu; J L Johnson
Journal:  Theor Appl Genet       Date:  1992-04       Impact factor: 5.699

5.  AFLP: a new technique for DNA fingerprinting.

Authors:  P Vos; R Hogers; M Bleeker; M Reijans; T van de Lee; M Hornes; A Frijters; J Pot; J Peleman; M Kuiper
Journal:  Nucleic Acids Res       Date:  1995-11-11       Impact factor: 16.971

6.  Rapid isolation of high molecular weight plant DNA.

Authors:  M G Murray; W F Thompson
Journal:  Nucleic Acids Res       Date:  1980-10-10       Impact factor: 16.971

7.  The transcriptional activator Opaque-2 controls the expression of a cytosolic form of pyruvate orthophosphate dikinase-1 in maize endosperms.

Authors:  M Maddaloni; G Donini; C Balconi; E Rizzi; P Gallusci; F Forlani; S Lohmer; R Thompson; F Salamini; M Motto
Journal:  Mol Gen Genet       Date:  1996-03-20

8.  The role of opaque2 in the control of lysine-degrading activities in developing maize endosperm.

Authors:  E L Kemper; G C Neto; F Papes; K C Moraes; A Leite; P Arruda
Journal:  Plant Cell       Date:  1999-10       Impact factor: 11.277

9.  MUTANT GENE THAT CHANGES PROTEIN COMPOSITION AND INCREASES LYSINE CONTENT OF MAIZE ENDOSPERM.

Authors:  E T MERTZ; L S BATES; O E NELSON
Journal:  Science       Date:  1964-07-17       Impact factor: 47.728

10.  Assessment of genome origins and genetic diversity in the genus Eleusine with DNA markers.

Authors:  S S Salimath; A C de Oliveira; I D Godwin; J L Bennetzen
Journal:  Genome       Date:  1995-08       Impact factor: 2.166
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  17 in total

Review 1.  Systems Biology for Smart Crops and Agricultural Innovation: Filling the Gaps between Genotype and Phenotype for Complex Traits Linked with Robust Agricultural Productivity and Sustainability.

Authors:  Anil Kumar; Rajesh Kumar Pathak; Sanjay Mohan Gupta; Vikram Singh Gaur; Dinesh Pandey
Journal:  OMICS       Date:  2015-10

2.  Association mapping of agro-morphological characters among the global collection of finger millet genotypes using genomic SSR markers.

Authors:  B Kalyana Babu; P K Agrawal; Dinesh Pandey; J P Jaiswal; Anil Kumar
Journal:  Mol Biol Rep       Date:  2014-05-27       Impact factor: 2.316

3.  Cross-genera transferability of rice and finger millet genomic SSRs to barnyard millet (Echinochloa spp.).

Authors:  B Kalyana Babu; Salej Sood; Dinesh Kumar; Anjeli Joshi; A Pattanayak; Lakshmi Kant; H D Upadhyaya
Journal:  3 Biotech       Date:  2018-01-24       Impact factor: 2.406

Review 4.  Microbial-assisted and genomic-assisted breeding: a two way approach for the improvement of nutritional quality traits in agricultural crops.

Authors:  Ajay Kumar Chandra; Amarjeet Kumar; Alka Bharati; Rini Joshi; Aparna Agrawal; Sumit Kumar
Journal:  3 Biotech       Date:  2019-11-26       Impact factor: 2.406

5.  Spermidine sprays alleviate the water deficit-induced oxidative stress in finger millet (Eleusine coracana L. Gaertn.) plants.

Authors:  Lakkakula Satish; Arockiam Sagina Rency; Manikandan Ramesh
Journal:  3 Biotech       Date:  2018-01-11       Impact factor: 2.406

Review 6.  Genetic diversity and genomic resources available for the small millet crops to accelerate a New Green Revolution.

Authors:  Travis L Goron; Manish N Raizada
Journal:  Front Plant Sci       Date:  2015-03-24       Impact factor: 5.753

7.  Tracing QTLs for Leaf Blast Resistance and Agronomic Performance of Finger Millet (Eleusine coracana (L.) Gaertn.) Genotypes through Association Mapping and in silico Comparative Genomics Analyses.

Authors:  M Ramakrishnan; S Antony Ceasar; V Duraipandiyan; K K Vinod; Krishnan Kalpana; N A Al-Dhabi; S Ignacimuthu
Journal:  PLoS One       Date:  2016-07-14       Impact factor: 3.240

8.  Assessment of genetic diversity, population structure and relationships in Indian and non-Indian genotypes of finger millet (Eleusine coracana (L.) Gaertn) using genomic SSR markers.

Authors:  M Ramakrishnan; S Antony Ceasar; V Duraipandiyan; N A Al-Dhabi; S Ignacimuthu
Journal:  Springerplus       Date:  2016-02-11

9.  Comparative genomics and association mapping approaches for blast resistant genes in finger millet using SSRs.

Authors:  B Kalyana Babu; Pandey Dinesh; Pawan K Agrawal; S Sood; C Chandrashekara; Jagadish C Bhatt; Anil Kumar
Journal:  PLoS One       Date:  2014-06-10       Impact factor: 3.240

Review 10.  Nutraceutical Value of Finger Millet [Eleusine coracana (L.) Gaertn.], and Their Improvement Using Omics Approaches.

Authors:  Anil Kumar; Mamta Metwal; Sanveen Kaur; Atul K Gupta; Swati Puranik; Sadhna Singh; Manoj Singh; Supriya Gupta; B K Babu; Salej Sood; Rattan Yadav
Journal:  Front Plant Sci       Date:  2016-06-29       Impact factor: 5.753
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