W Perng1, E Villamor2, M R Shroff3, J A Nettleton4, J R Pilsner5, Y Liu6, A V Diez-Roux2. 1. Department of Epidemiology, University of Michigan School of Public Health, Ann Arbor, MI, USA. Electronic address: wei.perng@gmail.com. 2. Department of Epidemiology, University of Michigan School of Public Health, Ann Arbor, MI, USA. 3. Center for Healthy Communities, Michigan Public Health Institute, Okemos, MI, USA. 4. Division of Epidemiology, Human Genetics, and Environmental Sciences, University of Texas Health Science Center, Houston, TX, USA. 5. Department of Environmental Health Science, School of Public Health and Health Sciences, University of Massachusetts, Amherst, MA, USA. 6. Sticht Center on Aging, Wake Forest University, Winston-Salem, NC, USA.
Abstract
BACKGROUND AND AIMS: DNA methylation of repetitive elements may explain the relations between dietary intake, hyperhomocysteinemia, and cardiovascular disease risk. We investigated associations of methyl micronutrient intake and plasma total homocysteine with LINE-1 and Alu methylation in a cross-sectional study of 987 adults aged 45-84 y who participated in the Multi-Ethnic Study of Atherosclerosis (MESA) Stress Study. METHODS AND RESULTS: DNA methylation was estimated using pyrosequencing technology. A 120-item food frequency questionnaire was used to ascertain daily intake of folate, vitamin B12, vitamin B6, zinc, and methionine. Plasma total homocysteine was quantified using a fluorescence polarization immunoassay. Associations of micronutrient intake and homocysteine with LINE-1 and Alu methylation were examined using linear regression. Adjusted differences in %5-methylated cytosines (%5 mC) were examined by categories of predictors using multivariable linear regression models. Intake of methyl-donor micronutrients was not associated with DNA methylation. After adjustment for covariates, each 3 μmol/L increment of homocysteine corresponded with 0.06 (-0.01, 0.13) %5 mC higher LINE-1 methylation. Additionally, BMI was positively associated with LINE-1 methylation (P trend = 0.03). Participants with BMI ≥ 40 kg/m² had 0.35 (0.03, 0.67) %5 mC higher LINE-1 than those with normal BMI. We also observed a 0.10 (0.02, 0.19) %5 mC difference in Alu methylation per 10 cm of height. These associations did not differ by sex. CONCLUSION: Dietary intake of methyl-donor micronutrients was not associated with measures of DNA methylation in our sample. However, higher BMI was related to higher LINE-1 methylation, and height was positively associated with Alu methylation.
BACKGROUND AND AIMS: DNA methylation of repetitive elements may explain the relations between dietary intake, hyperhomocysteinemia, and cardiovascular disease risk. We investigated associations of methyl micronutrient intake and plasma total homocysteine with LINE-1 and Alu methylation in a cross-sectional study of 987 adults aged 45-84 y who participated in the Multi-Ethnic Study of Atherosclerosis (MESA) Stress Study. METHODS AND RESULTS: DNA methylation was estimated using pyrosequencing technology. A 120-item food frequency questionnaire was used to ascertain daily intake of folate, vitamin B12, vitamin B6, zinc, and methionine. Plasma total homocysteine was quantified using a fluorescence polarization immunoassay. Associations of micronutrient intake and homocysteine with LINE-1 and Alu methylation were examined using linear regression. Adjusted differences in %5-methylated cytosines (%5 mC) were examined by categories of predictors using multivariable linear regression models. Intake of methyl-donor micronutrients was not associated with DNA methylation. After adjustment for covariates, each 3 μmol/L increment of homocysteine corresponded with 0.06 (-0.01, 0.13) %5 mC higher LINE-1 methylation. Additionally, BMI was positively associated with LINE-1 methylation (P trend = 0.03). Participants with BMI ≥ 40 kg/m² had 0.35 (0.03, 0.67) %5 mC higher LINE-1 than those with normal BMI. We also observed a 0.10 (0.02, 0.19) %5 mC difference in Alu methylation per 10 cm of height. These associations did not differ by sex. CONCLUSION: Dietary intake of methyl-donor micronutrients was not associated with measures of DNA methylation in our sample. However, higher BMI was related to higher LINE-1 methylation, and height was positively associated with Alu methylation.
Authors: N R M Buist; B Glenn; O Vugrek; C Wagner; S Stabler; R H Allen; I Pogribny; A Schulze; S H Zeisel; I Barić; S H Mudd Journal: J Inherit Metab Dis Date: 2006-05-30 Impact factor: 4.982
Authors: Anthony A Fryer; Tamer M Nafee; Khaled M K Ismail; William D Carroll; Richard D Emes; William E Farrell Journal: Epigenetics Date: 2009-08-18 Impact factor: 4.528
Authors: R A Jacob; D M Gretz; P C Taylor; S J James; I P Pogribny; B J Miller; S M Henning; M E Swendseid Journal: J Nutr Date: 1998-07 Impact factor: 4.798
Authors: Mahmoud Al Rifai; Andrew P DeFilippis; John W McEvoy; Michael E Hall; Ana Navas Acien; Miranda R Jones; Rachel Keith; Hoda S Magid; Carlos J Rodriguez; Graham R Barr; Emelia J Benjamin; Rose Marie Robertson; Aruni Bhatnagar; Michael J Blaha Journal: Atherosclerosis Date: 2017-01-19 Impact factor: 5.162
Authors: Pooja R Mandaviya; Roby Joehanes; Jennifer Brody; Juan E Castillo-Fernandez; Koen F Dekkers; Anh N Do; Mariaelisa Graff; Ismo K Hänninen; Toshiko Tanaka; Ester A L de Jonge; Jessica C Kiefte-de Jong; Devin M Absher; Stella Aslibekyan; Yolanda B de Rijke; Myriam Fornage; Dena G Hernandez; Mikko A Hurme; M Arfan Ikram; Paul F Jacques; Anne E Justice; Douglas P Kiel; Rozenn N Lemaitre; Michael M Mendelson; Vera Mikkilä; Ann Z Moore; Tess Pallister; Olli T Raitakari; Casper G Schalkwijk; Jin Sha; Eline P E Slagboom; Caren E Smith; Coen D A Stehouwer; Pei-Chien Tsai; André G Uitterlinden; Carla J H van der Kallen; Diana van Heemst; Donna K Arnett; Stefania Bandinelli; Jordana T Bell; Bastiaan T Heijmans; Terho Lehtimäki; Daniel Levy; Kari E North; Nona Sotoodehnia; Marleen M J van Greevenbroek; Joyce B J van Meurs; Sandra G Heil Journal: Am J Clin Nutr Date: 2019-08-01 Impact factor: 8.472