Literature DB >> 2447562

alpha-DNA. VI: Comparative study of alpha- and beta-anomeric oligodeoxyribonucleotides in hybridization to mRNA and in cell free translation inhibition.

C Gagnor1, J R Bertrand, S Thenet, M Lemaître, F Morvan, B Rayner, C Malvy, B Lebleu, J L Imbach, C Paoletti.   

Abstract

alpha and beta-anomeric d(G2T12G2) oligodeoxyribonucleotides were compared for their hybridization to rA12: the observed melting temperatures are 27 degrees C for beta-oligodeoxyribonucleotide/RNA hybrid and 53 degrees C for alpha-oligodeoxyribonucleotide/RNA. alpha-oligonucleotides with the four bases, complementary to natural mRNAs, were synthesized for the first time, labeled at their 5'-end with [32P] and used as probes in Northern blot experiments. In spite of these higher affinities for their target RNA's, they were unable to block translation of natural or synthetic mRNA's in rabbit reticulocyte lysate. We have studied the RNase H activity on model rA12:alpha- or beta-d(G2T12G2) hybrids or on mRNA:alpha- or beta-oligonucleotides hybrids. Specific hybridization protects RNA strech when using alpha-oligonucleotides but not beta-oligonucleotides. Thus, our results show the inability of RNase H to degrade RNA in alpha-oligodeoxyribonucleotides:RNA duplexes.

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Year:  1987        PMID： 2447562      PMCID： PMC339953          DOI： 10.1093/nar/15.24.10419

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  29 in total

1.  The use of single-stranded DNA and RNase H to promote quantitative 'hybrid arrest of translation' of mRNA/DNA hybrids in reticulocyte lysate cell-free translations.

Authors:  J Minshull; T Hunt
Journal:  Nucleic Acids Res       Date:  1986-08-26       Impact factor: 16.971

Review 2.  Redefining interferon: the interferon-like antiviral effects of certain cytokines (interleukin-1, interferon-beta 2, interferon-gamma) may be indirect or side effects.

Authors:  A Billiau
Journal:  Antiviral Res       Date:  1987-09       Impact factor: 5.970

3.  Enzymatic amplification of translation inhibition of rabbit beta-globin mRNA mediated by anti-messenger oligodeoxynucleotides covalently linked to intercalating agents.

Authors:  C Cazenave; N Loreau; N T Thuong; J J Toulmé; C Hélène
Journal:  Nucleic Acids Res       Date:  1987-06-25       Impact factor: 16.971

4.  Specific antiviral activity of a poly(L-lysine)-conjugated oligodeoxyribonucleotide sequence complementary to vesicular stomatitis virus N protein mRNA initiation site.

Authors:  M Lemaitre; B Bayard; B Lebleu
Journal:  Proc Natl Acad Sci U S A       Date:  1987-02       Impact factor: 11.205

5.  Inhibition of replication and expression of human T-cell lymphotropic virus type III in cultured cells by exogenous synthetic oligonucleotides complementary to viral RNA.

Authors:  P C Zamecnik; J Goodchild; Y Taguchi; P S Sarin
Journal:  Proc Natl Acad Sci U S A       Date:  1986-06       Impact factor: 11.205

6.  Solid phase synthesis of oligoribonucleotides using o-nitrobenzyl protection of 2'-hydroxyl via a phosphite triester approach.

Authors:  T Tanaka; S Tamatsukuri; M Ikehara
Journal:  Nucleic Acids Res       Date:  1986-08-11       Impact factor: 16.971

7.  Translation arrest by oligodeoxynucleotides complementary to mRNA coding sequences yields polypeptides of predetermined length.

Authors:  M T Haeuptle; R Frank; B Dobberstein
Journal:  Nucleic Acids Res       Date:  1986-02-11       Impact factor: 16.971

8.  alpha-DNA. I. Synthesis, characterization by high field 1H-NMR, and base-pairing properties of the unnatural hexadeoxyribonucleotide alpha-[d(CpCpTpTpCpC)] with its complement beta-[d(GpGpApApGpG)].

Authors:  F Morvan; B Rayner; J L Imbach; D K Chang; J W Lown
Journal:  Nucleic Acids Res       Date:  1986-06-25       Impact factor: 16.971

9.  Nucleotide sequence of the rightward operator of phage lambda.

Authors:  T Maniatis; A Jeffrey; D G Kleid
Journal:  Proc Natl Acad Sci U S A       Date:  1975-03       Impact factor: 11.205

10.  B cell growth modulating and differentiating activity of recombinant human 26-kd protein (BSF-2, HuIFN-beta 2, HPGF).

Authors:  P Poupart; P Vandenabeele; S Cayphas; J Van Snick; G Haegeman; V Kruys; W Fiers; J Content
Journal:  EMBO J       Date:  1987-05       Impact factor: 11.598
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  27 in total

1.  Inhibition of translation initiation by antisense oligonucleotides via an RNase-H independent mechanism.

Authors:  C Boiziau; R Kurfurst; C Cazenave; V Roig; N T Thuong; J J Toulmé
Journal:  Nucleic Acids Res       Date:  1991-03-11       Impact factor: 16.971

2.  Can hammerhead ribozymes be efficient tools to inactivate gene function?

Authors:  E Bertrand; R Pictet; T Grange
Journal:  Nucleic Acids Res       Date:  1994-02-11       Impact factor: 16.971

3.  Selective inhibition of cell-free translation by oligonucleotides targeted to a mRNA hairpin structure.

Authors:  R Le Tinévez; R K Mishra; J J Toulmé
Journal:  Nucleic Acids Res       Date:  1998-05-15       Impact factor: 16.971

4.  RNase H cleavage of RNA hybridized to oligonucleotides containing methylphosphonate, phosphorothioate and phosphodiester bonds.

Authors:  P J Furdon; Z Dominski; R Kole
Journal:  Nucleic Acids Res       Date:  1989-11-25       Impact factor: 16.971

5.  Effects of oligo sequence and chemistry on the efficiency of oligodeoxyribonucleotide-mediated mRNA cleavage.

Authors:  C Baker; D Holland; M Edge; A Colman
Journal:  Nucleic Acids Res       Date:  1990-06-25       Impact factor: 16.971

6.  In vitro inhibition of hepatitis C virus gene expression by chemically modified antisense oligodeoxynucleotides.

Authors:  O Vidalin; M E Major; B Rayner; J L Imbach; C Trépo; G Inchauspé
Journal:  Antimicrob Agents Chemother       Date:  1996-10       Impact factor: 5.191

7.  The binding of an antisense oligonucleotide to a hairpin structure via triplex formation inhibits chemical and biological reactions.

Authors:  E Brossalina; E Pascolo; J J Toulmé
Journal:  Nucleic Acids Res       Date:  1993-12-11       Impact factor: 16.971

Review 8.  Antisense oncogene and tumor suppressor gene therapy of cancer.

Authors:  W W Zhang
Journal:  J Mol Med (Berl)       Date:  1996-04       Impact factor: 4.599

9.  Mechanisms of the inhibition of reverse transcription by antisense oligonucleotides.

Authors:  C Boiziau; N T Thuong; J J Toulmé
Journal:  Proc Natl Acad Sci U S A       Date:  1992-01-15       Impact factor: 11.205

10.  Synthesis and base-pairing properties of the nuclease-resistant alpha-anomeric dodecaribonucleotide alpha-[r(UCUUAACCCACA)].

Authors:  F Debart; B Rayner; G Degols; J L Imbach
Journal:  Nucleic Acids Res       Date:  1992-03-25       Impact factor: 16.971
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