| Literature DB >> 24472437 |
Jeong-Woo Kang1, Yun Sun Park1, Dong Hun Lee1, Man Sub Kim1, Yesol Bak1, Sun Young Ham1, Su Ho Park1, Heejong Kim1, Joong Hoon Ahn1, Jin Tae Hong2, Do-Young Yoon3.
Abstract
IL-32 has been studied for its pleiotropic effects ranging from host immune responses to cell differentiation. Although several IL-32 isoforms have been characterized for their effects on cells, the roles of the others remain unclear. We previously reported that IL-32δ interacted with IL-32β and inhibited IL-32β-mediated IL-10 production. Thus, we performed comprehensive analyses to reveal more interactions between IL-32 isoforms in this study. We screened the interactions of 81 combinations of nine IL-32 isoforms by using a yeast two-hybrid assay, which identified 13 heterodimeric interactions. We verified these results by using reciprocal immunoprecipitation assays and reconfirmed 10 interactions, and presented the interaction network map between IL-32 isoforms. Our data suggest that IL-32 may have diverse intracellular effects through the interactions with its different isoforms.Entities:
Keywords: IL-32; Immunoprecipitation; Interaction map; Isoform interaction; Yeast two-hybrid
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Year: 2014 PMID： 24472437 DOI： 10.1016/j.biochi.2014.01.013
Source DB: PubMed Journal: Biochimie ISSN： 0300-9084 Impact factor: 4.079