Literature DB >> 24470248

A new procedure for the purification of spinach leaf photosynthetic fructose-1,6-bisphosphatase by affinity chromatography on mercaptoethylamine-Sepharose.

A Plá1, A Chueca, J López-Gorgé.   

Abstract

A new purification procedure for spinach leaf fructose-1,6-bisphosphatase is proposed, which includes the use of affinity chromatography on mercaptoethylamine-Sepharose. A homogeneous preparation of the enzyme can be obtained in 48 hr, with a specific activity of 67 U/mg and a yield of 23%. The method may also be useful for the purification of other thioredoxin-activated chloroplast enzymes.

Entities:  

Year:  1981        PMID： 24470248     DOI： 10.1007/BF00056266

Source DB:  PubMed          Journal:  Photosynth Res        ISSN： 0166-8595            Impact factor:   3.573


  14 in total

1.  Identification of a protein factor involved in dithiothreitol activation of NADP malate dehydrogenase from French bean leaves.

Authors:  J P Jacquot; J Vidal; P Gadal
Journal:  FEBS Lett       Date:  1976-12-01       Impact factor: 4.124

2.  Efficient purification and molecular properties of spinach chloroplast fructose 1,6-bisphosphatase.

Authors:  G Zimmermann; G J Kelly; E Latzko
Journal:  Eur J Biochem       Date:  1976-11-15

3.  DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors:  B J DAVIS
Journal:  Ann N Y Acad Sci       Date:  1964-12-28       Impact factor: 5.691

4.  Hexosediphosphatase from spinach chloroplasts: purification, crystallization and some properties.

Authors:  A M El-Badry
Journal:  Biochim Biophys Acta       Date:  1974-02-22

5.  Purification and properties of spinach leaf cytoplasmic fructose-1,6-bisphosphatase.

Authors:  G Zimmermann; G J Kelly; E Latzko
Journal:  J Biol Chem       Date:  1978-09-10       Impact factor: 5.157

6.  Ferredoxin-activated fructose diphosphatase of spinach chloroplasts. Resolution of the system, properties of the alkaline fructose diphosphatase component, and physiological significance of the ferredoxin-linked activation.

Authors:  B B Buchanan; P Schürmann; P P Kalberer
Journal:  J Biol Chem       Date:  1971-10-10       Impact factor: 5.157

7.  FDPases of the castor bean endosperm and leaf: properties and partial purification.

Authors:  J Scala; C Patrick; G Macbeth
Journal:  Arch Biochem Biophys       Date:  1968-09-20       Impact factor: 4.013

8.  D-Fructose-1,6-diphosphatase of navy bean (Phaseolus vulgaris L.) leaves.

Authors:  J Scala; G Ketner; W H Jyung
Journal:  Arch Biochem Biophys       Date:  1969-04       Impact factor: 4.013

9.  Thioredoxin/fructose-1,6-bisphosphatase affinity in the enzyme activation by the ferredoxin-thioredoxin system.

Authors:  A Pla; J Lopez-Gorge
Journal:  Biochim Biophys Acta       Date:  1981-06-12

10.  Activation of Chloroplast NADP-linked Glyceraldehyde-3-Phosphate Dehydrogenase by the Ferredoxin/Thioredoxin System.

Authors:  R A Wolosiuk; B B Buchanan
Journal:  Plant Physiol       Date:  1978-04       Impact factor: 8.340
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  3 in total

1.  Purification and properties of pea (Pisum sativum L.) thioredoxin f, a plant thioredoxin with unique features in the activation of chloroplast fructose-1,6-bisphosphatase.

Authors:  F E Prado; J J Lázaro; R Hermoso; A Chueca; J L Gorgé
Journal:  Planta       Date:  1992-10       Impact factor: 4.116

2.  An immunological method for quantitative determination of photosynthetic fructose-1,6-bisphosphatase in leaf crude extracts.

Authors:  R Hermoso; A Chueca; J J Lazaro; J Lopez-Gorge
Journal:  Photosynth Res       Date:  1987-01       Impact factor: 3.573

3.  Cloning, structure and expression of a pea cDNA clone coding for a photosynthetic fructose-1,6-bisphosphatase with some features different from those of the leaf chloroplast enzyme.

Authors:  J L Carrasco; A Chueca; F E Prado; R Hermoso; J J Lázaro; J L Ramos; M Sahrawy; J López Gorgé
Journal:  Planta       Date:  1994       Impact factor: 4.116
  3 in total

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