| Literature DB >> 24470054 |
Yi-Ming Li1, Yi-Tong Li, Man Pan, Xiu-Qi Kong, Yi-Chao Huang, Zhang-Yong Hong, Lei Liu.
Abstract
Sortase-mediated hydrazinolysis of proteins with hydrazine or its derivatives was developed for the production of recombinant protein hydrazides. This process provides an alternative approach for protein semisynthesis through the use of recombinant protein hydrazides as thioester surrogates. It also provides an alternative method for C-terminal modification of proteins with functional units as well as for the preparation of C-to-C fusion proteins.Entities:
Keywords: hydrazones; hydrolysis; ligation; protein modification; proteins
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Year: 2014 PMID: 24470054 DOI: 10.1002/anie.201310010
Source DB: PubMed Journal: Angew Chem Int Ed Engl ISSN: 1433-7851 Impact factor: 15.336