Hideaki Nakatsuji1, Ken Kishida2, Ryohei Sekimoto1, Noriyuki Komura1, Shinji Kihara1, Tohru Funahashi3, Iichiro Shimomura1. 1. Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka, Japan. 2. Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka, Japan. Electronic address: kkishida@imed2.med.osaka-u.ac.jp. 3. Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka, Japan; Department of Metabolism and Atherosclerosis, Graduate School of Medicine, Osaka University, Osaka, Japan.
Abstract
OBJECTIVE: Adipose tissue inflammation plays an important role in the pathogenesis of obesity-associated complications, such as atherosclerosis. Adiponectin secreted from adipocytes has various beneficial effects including anti-inflammatory effect. Obesity often presents with hypoadiponectinemia. However, the mechanism and adiponectin movement in obesity remain uncharacterized. Here we investigated tissue distribution of adiponectin protein in lean and obese mice. METHODS: Adiponectin protein levels were evaluated by enzyme-linked immunosorbent assay and western blotting. Adipose tissues were fractionated into mature adipocyte fraction (MAF) and stromal vascular fraction (SVF). RESULTS: Adiponectin protein was detected not only in MAF but also in SVF, which lacks adiponectin mRNA expression, of adipose tissue remarkably. SVF adiponectin protein level was higher in obese mice than in lean mice. The mechanism of adiponectin accumulation was investigated in adiponectin-deficient (APN-KO) mice after injection of plasma from wild-type mice. These mice showed accumulation of exogenous adiponectin, which derived from wild type mice, in adipose tissues, and the adiponectin was more observed in SVF of diet induced obese APN-KO mice than lean APN-KO mice. Among the adiponectin binding proteins, T-cadherin mRNA and protein levels in SVF of obese mice were remarkably higher than in lean mice. Oxidative stress levels were also significantly higher in SVF of obese mice than lean mice. Mechanistically, H2O2 up-regulated T-cadherin mRNA level in murine macrophages. CONCLUSIONS: The results demonstrated adiponectin targets to adipose SVF of obese mice. These findings should shed a new light on the pathology of adipose tissue inflammation and hypoadiponectinemia of obesity.
OBJECTIVE: Adipose tissue inflammation plays an important role in the pathogenesis of obesity-associated complications, such as atherosclerosis. Adiponectin secreted from adipocytes has various beneficial effects including anti-inflammatory effect. Obesity often presents with hypoadiponectinemia. However, the mechanism and adiponectin movement in obesity remain uncharacterized. Here we investigated tissue distribution of adiponectin protein in lean and obesemice. METHODS:Adiponectin protein levels were evaluated by enzyme-linked immunosorbent assay and western blotting. Adipose tissues were fractionated into mature adipocyte fraction (MAF) and stromal vascular fraction (SVF). RESULTS:Adiponectin protein was detected not only in MAF but also in SVF, which lacks adiponectin mRNA expression, of adipose tissue remarkably. SVF adiponectin protein level was higher in obesemice than in lean mice. The mechanism of adiponectin accumulation was investigated in adiponectin-deficient (APN-KO) mice after injection of plasma from wild-type mice. These mice showed accumulation of exogenous adiponectin, which derived from wild type mice, in adipose tissues, and the adiponectin was more observed in SVF of diet induced obese APN-KOmice than lean APN-KO mice. Among the adiponectin binding proteins, T-cadherin mRNA and protein levels in SVF of obesemice were remarkably higher than in lean mice. Oxidative stress levels were also significantly higher in SVF of obesemice than lean mice. Mechanistically, H2O2 up-regulated T-cadherin mRNA level in murine macrophages. CONCLUSIONS: The results demonstrated adiponectin targets to adipose SVF of obesemice. These findings should shed a new light on the pathology of adipose tissue inflammation and hypoadiponectinemia of obesity.
Authors: Xiaowen Liu; Nikolaos Perakakis; Huizhi Gong; John P Chamberland; Mary T Brinkoetter; Ole-Petter R Hamnvik; Christos S Mantzoros Journal: Metabolism Date: 2016-09-22 Impact factor: 8.694
Authors: Maria Luisa Justo; Carmen Claro; Maximilian Zeyda; Thomas M Stulnig; María Dolores Herrera; Rosalía Rodríguez-Rodríguez Journal: Eur J Nutr Date: 2015-08-13 Impact factor: 5.614