AIMS: Focal adhesions have been associated with poor prognosis in multiple cancer types, but their prognostic value in diffuse large B-cell lymphoma (DLBCL) has not been evaluated. The aim of this study was to investigate the expression patterns and the prognostic value of the focal adhesion proteins FAK, Pyk2, p130Cas and HEF1 in DLBCL. METHODS AND RESULTS: Focal adhesion protein expression was examined using immunohistochemistry in normal lymphoid tissues and in 60 DLBCL patient samples. Kaplan-Meier survival and Cox regression analysis were performed to evaluate the correlation of focal adhesion protein expression with patient prognosis. FAK, Pyk2, p130Cas and HEF1 expression was mostly found in the germinal centres of normal human lymphoid tissues. When assessed in DLBCL samples, FAK, Pyk2, p130Cas and HEF1 were highly expressed in 45%, 34%, 42% and 45% of the samples, respectively. Multivariate Cox analysis revealed that decreased FAK expression was a significant independent predictor of poorer disease outcome. CONCLUSIONS: FAK expression is an independent prognostic factor in DLBCL. Our results suggest that the addition of FAK immunostaining to the current immunohistochemical algorithms may facilitate risk stratification of DLBCL patients.
AIMS: Focal adhesions have been associated with poor prognosis in multiple cancer types, but their prognostic value in diffuse large B-cell lymphoma (DLBCL) has not been evaluated. The aim of this study was to investigate the expression patterns and the prognostic value of the focal adhesion proteins FAK, Pyk2, p130Cas and HEF1 in DLBCL. METHODS AND RESULTS: Focal adhesion protein expression was examined using immunohistochemistry in normal lymphoid tissues and in 60 DLBCL patient samples. Kaplan-Meier survival and Cox regression analysis were performed to evaluate the correlation of focal adhesion protein expression with patient prognosis. FAK, Pyk2, p130Cas and HEF1 expression was mostly found in the germinal centres of normal human lymphoid tissues. When assessed in DLBCL samples, FAK, Pyk2, p130Cas and HEF1 were highly expressed in 45%, 34%, 42% and 45% of the samples, respectively. Multivariate Cox analysis revealed that decreased FAK expression was a significant independent predictor of poorer disease outcome. CONCLUSIONS:FAK expression is an independent prognostic factor in DLBCL. Our results suggest that the addition of FAK immunostaining to the current immunohistochemical algorithms may facilitate risk stratification of DLBCL patients.
Authors: Emilia L Lim; Diane L Trinh; David W Scott; Andy Chu; Martin Krzywinski; Yongjun Zhao; A Gordon Robertson; Andrew J Mungall; Jacqueline Schein; Merrill Boyle; Anja Mottok; Daisuke Ennishi; Nathalie A Johnson; Christian Steidl; Joseph M Connors; Ryan D Morin; Randy D Gascoyne; Marco A Marra Journal: Genome Biol Date: 2015-01-29 Impact factor: 13.583
Authors: Teresa Gómez Del Pulgar; Arancha Cebrián; Maria Jesús Fernández-Aceñero; Aurea Borrero-Palacios; Laura Del Puerto-Nevado; Javier Martínez-Useros; Juan Pablo Marín-Arango; Cristina Caramés; Ricardo Vega-Bravo; María Rodríguez-Remírez; Marlid Cruz-Ramos; Félix Manzarbeitia; Jesús García-Foncillas Journal: J Cell Mol Med Date: 2016-05-12 Impact factor: 5.310
Authors: Marina Roy-Luzarraga; Tarek Abdel-Fatah; Louise E Reynolds; Andrew Clear; Joseph G Taylor; John G Gribben; Stephen Chan; Louise Jones; Kairbaan Hodivala-Dilke Journal: JAMA Netw Open Date: 2020-10-01