Literature DB >> 24466488

Pulsetrain-burst mode, ultrafast-laser interactions with 3D viable cell cultures as a model for soft biological tissues.

Zuoming Qian1, Aghapi Mordovanakis2, Joshua E Schoenly1, Andrés Covarrubias1, Yuanfeng Feng1, Lothar Lilge3, Robin S Marjoribanks1.   

Abstract

A 3D living-cell culture in hydrogel has been developed as a standardized low-tensile-strength tissue proxy for study of ultrafast, pulsetrain-burst laser-tissue interactions. The hydrogel is permeable to fluorescent biomarkers and optically transparent, allowing viable and necrotic cells to be imaged in 3D by confocal microscopy. Good cell-viability allowed us to distinguish between typical cell mortality and delayed subcellular tissue damage (e.g., apoptosis and DNA repair complex formation), caused by laser irradiation. The range of necrosis depended on laser intensity, but not on pulsetrain-burst duration. DNA double-strand breaks were quantified, giving a preliminary upper limit for genetic damage following laser treatment.

Entities:  

Keywords:  (140.3440) Laser-induced breakdown; (160.1435) Biomaterials; (170.1020) Ablation of tissue; (170.2520) Fluorescence microscopy; (320.7090) Ultrafast lasers; (350.3390) Laser materials processing

Year:  2013        PMID： 24466488      PMCID： PMC3891333          DOI： 10.1364/BOE.5.000208

Source DB:  PubMed          Journal:  Biomed Opt Express        ISSN： 2156-7085            Impact factor:   3.732


  34 in total

1.  Selective photothermolysis of lipid-rich tissues: a free electron laser study.

Authors:  R Rox Anderson; William Farinelli; Hans Laubach; Dieter Manstein; Anna N Yaroslavsky; Joseph Gubeli; Kevin Jordan; George R Neil; Michelle Shinn; Walter Chandler; Gwyn P Williams; Steven V Benson; David R Douglas; H F Dylla
Journal:  Lasers Surg Med       Date:  2006-12       Impact factor: 4.025

2.  Micromachining of bulk glass with bursts of femtosecond laser pulses at variable repetition rates.

Authors:  Rafael R Gattass; Loren R Cerami; Eric Mazur
Journal:  Opt Express       Date:  2006-06-12       Impact factor: 3.894

3.  Dynamic mechanical stimulations induce anisotropy and improve the tensile properties of engineered tissues produced without exogenous scaffolding.

Authors:  Robert Gauvin; Rémi Parenteau-Bareil; Danielle Larouche; Hugo Marcoux; Francis Bisson; Adrien Bonnet; François A Auger; Stéphane Bolduc; Lucie Germain
Journal:  Acta Biomater       Date:  2011-05-30       Impact factor: 8.947

Review 4.  Femtosecond cataract surgery: transitioning to laser cataract.

Authors:  Gerard Sutton; Shveta Jindal Bali; Chris Hodge
Journal:  Curr Opin Ophthalmol       Date:  2013-01       Impact factor: 3.761

5.  Ultrashort pulsed laser (USPL) application in dentistry: basic investigations of ablation rates and thresholds on oral hard tissue and restorative materials.

Authors:  Florian Schelle; Sebastian Polz; Hatim Haloui; Andreas Braun; Claudia Dehn; Matthias Frentzen; Jörg Meister
Journal:  Lasers Med Sci       Date:  2013-04-23       Impact factor: 3.161

6.  A novel, sensitive fluorometric staining technique for the detection of DNA in RNA preparations.

Authors:  R Mocharla; H Mocharla; M E Hodes
Journal:  Nucleic Acids Res       Date:  1987-12-23       Impact factor: 16.971

7.  Topical delivery and photodynamic evaluation of a multivesicular liposomal Rose Bengal.

Authors:  Maha Fadel M Ali
Journal:  Lasers Med Sci       Date:  2010-12-01       Impact factor: 3.161

8.  Two-photon microscopy-guided femtosecond-laser photoablation of avian cardiogenesis: noninvasive creation of localized heart defects.

Authors:  Huseyin C Yalcin; Akshay Shekhar; Nozomi Nishimura; Ajinkya A Rane; Chris B Schaffer; Jonathan T Butcher
Journal:  Am J Physiol Heart Circ Physiol       Date:  2010-08-13       Impact factor: 4.733

9.  Hydrogel-filled polylactide porous scaffolds for cartilage tissue engineering.

Authors:  Yihong Gong; Lijuan He; Jun Li; Qingliang Zhou; Zuwei Ma; Changyou Gao; Jiacong Shen
Journal:  J Biomed Mater Res B Appl Biomater       Date:  2007-07       Impact factor: 3.368

10.  First clinical results with the femtosecond neodynium-glass laser in refractive surgery.

Authors:  Imola Ratkay-Traub; Istvan E Ferincz; Tibor Juhasz; Ron M Kurtz; Ronald R Krueger
Journal:  J Refract Surg       Date:  2003 Mar-Apr       Impact factor: 3.573
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  3 in total

1.  Dynamic absorption and scattering of water and hydrogel during high-repetition-rate (>100 MHz) burst-mode ultrafast-pulse laser ablation.

Authors:  Zuoming Qian; Andrés Covarrubias; Alexander W Grindal; Margarete K Akens; Lothar Lilge; Robin S Marjoribanks
Journal:  Biomed Opt Express       Date:  2016-05-23       Impact factor: 3.732

Review 2.  Fundamentals of Laser-Based Hydrogel Degradation and Applications in Cell and Tissue Engineering.

Authors:  Shantanu Pradhan; Keely A Keller; John L Sperduto; John H Slater
Journal:  Adv Healthc Mater       Date:  2017-10-24       Impact factor: 9.933

3.  Subsurface ablation of atherosclerotic plaque using ultrafast laser pulses.

Authors:  Thomas Lanvin; Donald B Conkey; Aurelien Frobert; Jeremy Valentin; Jean-Jacques Goy; Stéphane Cook; Marie-Noelle Giraud; Demetri Psaltis
Journal:  Biomed Opt Express       Date:  2015-06-16       Impact factor: 3.732
  3 in total

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