Identification of functional regions in the C-terminal domain of Escherichia coli ribosomal protein S1 using monoclonal antibodies.

Monoclonal antibodies specific for defined regions of E. coli ribosomal protein S1 were used in a R17 mRNA-directed protein synthesis assay to reveal functionally important sites of the protein. Two distinct sites for mRNA binding were identified in the regions 349-437 and 438-547 located in the C-terminal domain of protein S1.