Literature DB >> 2446526

Silver staining of native and denatured eucaryotic DNA in agarose gels.

M Gottlieb1, M Chavko.   

Abstract

A modified method of silver staining for native and denatured eucaryotic DNA in 1% agarose gel is described. This method is at least fivefold more sensitive than ethidium bromide staining, with a detection limit of 2.5 ng for total DNA. The calibration curve is linear within the range 5-30 ng of single-stranded and double-stranded DNA. This method is especially advantageous for electrophoretic assessment of DNA molecular weights.

Entities:  

Mesh：

Substances：

Year:  1987        PMID： 2446526     DOI： 10.1016/0003-2697(87)90197-7

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  13 in total

1.  Specific isoforms of plasminogen in patients with prostate cancer.

Authors:  D I Vodolazhsky; E F Shin; A Yu Golikov; T N Belova; D V Zimakov; E N Cherkasova; N V Boyko; M I Kogan; M B Chibichian; S A Moshkovsky; D G Matishov
Journal:  Dokl Biol Sci       Date:  2011-07-05

2.  Site-directed activation of calpain is promoted by a membrane-associated natural activator protein.

Authors:  F Salamino; R De Tullio; P Mengotti; P L Viotti; E Melloni; S Pontremoli
Journal:  Biochem J       Date:  1993-02-15       Impact factor: 3.857

3.  Pta1, a component of yeast CF II, is required for both cleavage and poly(A) addition of mRNA precursor.

Authors:  J Zhao; M Kessler; S Helmling; J P O'Connor; C Moore
Journal:  Mol Cell Biol       Date:  1999-11       Impact factor: 4.272

4.  Complementary DNAs encoding eukaryotic-type cytidine-5'-diphosphate-diacylglycerol synthases of two plant species.

Authors:  J Kopka; M Ludewig; B Müller-Röber
Journal:  Plant Physiol       Date:  1997-03       Impact factor: 8.340

5.  Enzymes of the Primary Phosphatidylethanolamine Biosynthetic Pathway in Postgermination Castor Bean Endosperm (Developmental Profiles and Partial Purification of the Mitochondrial CTP:Ethanolaminephosphate Cytidylyltransferase).

Authors:  F Tang; T S Moore
Journal:  Plant Physiol       Date:  1997-12       Impact factor: 8.340

6.  Novel microsatellite markers and single nucleotide polymorphisms refine the tylosis with oesophageal cancer (TOC) minimal region on 17q25 to 42.5 kb: sequencing does not identify the causative gene.

Authors:  Joanne E Langan; Charlotte G Cole; Elisabeth J Huckle; Shaun Byrne; Fiona E McRonald; Lynn Rowbottom; Anthony Ellis; Joan M Shaw; Irene M Leigh; David P Kelsell; Ian Dunham; John K Field; Janet M Risk
Journal:  Hum Genet       Date:  2004-03-09       Impact factor: 4.132

7.  Identification of rat liver phosphatidylinositol synthase as a 21 kDa protein.

Authors:  M E Monaco; M Feldman; D L Kleinberg
Journal:  Biochem J       Date:  1994-11-15       Impact factor: 3.857

8.  Adsorption of serum alpha-1-microglobulin onto biomaterials.

Authors:  M Santin; M Cannas; M A Wassall; S P Denyer
Journal:  J Mater Sci Mater Med       Date:  1998-03       Impact factor: 3.896

9.  Randomized, single-blind, placebo-controlled study of topical application of the immune response modulator resiquimod in healthy adults.

Authors:  Daniel N Sauder; Michael H Smith; Therese Senta-McMillian; Inmaculada Soria; Tze-Chiang Meng
Journal:  Antimicrob Agents Chemother       Date:  2003-12       Impact factor: 5.191

10.  Isolation of a yeast protein kinase that is activated by the protein encoded by SRP1 (Srp1p) and phosphorylates Srp1p complexed with nuclear localization signal peptides.

Authors:  Y Azuma; M M Tabb; L Vu; M Nomura
Journal:  Proc Natl Acad Sci U S A       Date:  1995-05-23       Impact factor: 11.205
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.