BACKGROUND: Sepsis in neonates hospitalized in the neonatal intensive care unit is a global problem and is a significant contributor to morbidity and mortality. Neutrophil surface CD64, the high-affinity Fc receptor, is quantitatively up-regulated during infection and sepsis. OBJECTIVE: Our goal in this prospective study was to measure the neutrophil CD64 in blood as an adjunct to our previously validated hematologic scoring system for detecting neonatal sepsis. METHODS: A prospective study enrolled newborns with documented sepsis (n = 25), clinical sepsis (n = 25), and control newborns (n = 25). C-reactive protein, neutrophil CD64, complete blood counts, and blood cultures were taken. Neutrophil CD64 was analyzed by flow cytometry. RESULTS: CD64 was significantly elevated in the groups with documented and clinical sepsis (P < 0.001). CD64 had a sensitivity of 96%, a specificity of 100%, a positive predictive value of 96.2%, and a negative predictive value of 100% with a cutoff value of 45.8% and 46.0% in the confirmed and the clinical sepsis groups, respectively. CONCLUSIONS: CD64 expression on neutrophils increases significantly in neonates with sepsis and can be considered a useful diagnostic marker for early diagnosis of neonatal infection as a single determination compared with other inflammatory markers.
BACKGROUND:Sepsis in neonates hospitalized in the neonatal intensive care unit is a global problem and is a significant contributor to morbidity and mortality. Neutrophil surface CD64, the high-affinity Fc receptor, is quantitatively up-regulated during infection and sepsis. OBJECTIVE: Our goal in this prospective study was to measure the neutrophil CD64 in blood as an adjunct to our previously validated hematologic scoring system for detecting neonatal sepsis. METHODS: A prospective study enrolled newborns with documented sepsis (n = 25), clinical sepsis (n = 25), and control newborns (n = 25). C-reactive protein, neutrophil CD64, complete blood counts, and blood cultures were taken. Neutrophil CD64 was analyzed by flow cytometry. RESULTS:CD64 was significantly elevated in the groups with documented and clinical sepsis (P < 0.001). CD64 had a sensitivity of 96%, a specificity of 100%, a positive predictive value of 96.2%, and a negative predictive value of 100% with a cutoff value of 45.8% and 46.0% in the confirmed and the clinical sepsis groups, respectively. CONCLUSIONS:CD64 expression on neutrophils increases significantly in neonates with sepsis and can be considered a useful diagnostic marker for early diagnosis of neonatal infection as a single determination compared with other inflammatory markers.
Authors: Florian Kipfmueller; Jessica Schneider; Julia Prusseit; Ioanna Dimitriou; Berndt Zur; Axel R Franz; Peter Bartmann; Andreas Mueller Journal: PLoS One Date: 2015-04-20 Impact factor: 3.240
Authors: Ning Xu; Juan Chen; Xin Chang; Jingwen Zhang; Qinghua Liu; Aljun Li; Dianjie Lin Journal: Ann Saudi Med Date: 2016 Jan-Feb Impact factor: 1.526
Authors: Heba E Hashem; Sherin A El Masry; Amira M Mokhtar; Eman A Ismail; Noureldin M Abdelaal Journal: Biomed Res Int Date: 2020-08-07 Impact factor: 3.411