Seung Hyeun Lee1, Sang Hoon Lee2, Chul Hwan Kim3, Kyung Suk Yang4, Eun Joo Lee5, Kyung Hoon Min5, Gyu Young Hur5, Seung Heon Lee5, Sung Yong Lee5, Je Hyeong Kim5, Chol Shin5, Jae Jeong Shim5, Kwang Ho In5, Kyung Ho Kang5, Sang Yeub Lee6. 1. Division of Respiratory and Critical Care Medicine, Department of Internal Medicine, KEPCO Medical Center, Seoul, Republic of Korea. 2. Department of Anatomy, Korea University College of Medicine, Seoul, Republic of Korea. 3. Department of Pathology, Korea University College of Medicine, Seoul, Republic of Korea. 4. Department of Biostatistics, Korea University College of Medicine, Seoul, Republic of Korea. 5. Division of Respiratory and Critical Care Medicine, Department of Internal Medicine, Korea University College of Medicine, Seoul, Republic of Korea. 6. Division of Respiratory and Critical Care Medicine, Department of Internal Medicine, Korea University College of Medicine, Seoul, Republic of Korea. Electronic address: pulsy@korea.ac.kr.
Abstract
OBJECTIVES: Vascular endothelial growth factor (VEGF) seems to be involved in the pathogenesis of chronic obstructive pulmonary disease (COPD), but its site-specific expression in lung tissue and the relationship with hypoxia inducible factor-1 alpha (HIF-1α) expression in chronic bronchitis (CB) type COPD have not been studied. DESIGN AND METHODS: We evaluated the expression of VEGF and its receptors in various compartments of lung tissue in three groups: non-smokers with normal lung function (non-smokers, n=10), smokers without COPD (healthy smokers, n=10) and smokers with CB (CB, n=10), using immunohistochemical staining and Western blotting. The expression of HIF-1α was assessed by enzyme-linked immunosorbent assay. RESULTS: Compared with healthy smokers, VEGF expression in CB was significantly increased in bronchiolar epithelium, vascular endothelium and vascular smooth muscle (p<0.05). VEGF receptor (VEGFR)-2 expression in CB was also increased in bronchiolar smooth muscle, vascular endothelium and vascular smooth muscle compared with healthy smokers (p<0.05). The level of HIF-1α was increased in CB compared with healthy smokers and positively correlated with those of VEGF (r=0.64, p<0.05). CONCLUSION: VEGF and VEGFR-2 expressions were up-regulated in CB and increased expression of VEGF was related with HIF-1α. HIF-1α-regulated VEGF overexpression may be a characteristic of chronic bronchitis.
OBJECTIVES:Vascular endothelial growth factor (VEGF) seems to be involved in the pathogenesis of chronic obstructive pulmonary disease (COPD), but its site-specific expression in lung tissue and the relationship with hypoxia inducible factor-1 alpha (HIF-1α) expression in chronic bronchitis (CB) type COPD have not been studied. DESIGN AND METHODS: We evaluated the expression of VEGF and its receptors in various compartments of lung tissue in three groups: non-smokers with normal lung function (non-smokers, n=10), smokers without COPD (healthy smokers, n=10) and smokers with CB (CB, n=10), using immunohistochemical staining and Western blotting. The expression of HIF-1α was assessed by enzyme-linked immunosorbent assay. RESULTS: Compared with healthy smokers, VEGF expression in CB was significantly increased in bronchiolar epithelium, vascular endothelium and vascular smooth muscle (p<0.05). VEGF receptor (VEGFR)-2 expression in CB was also increased in bronchiolar smooth muscle, vascular endothelium and vascular smooth muscle compared with healthy smokers (p<0.05). The level of HIF-1α was increased in CB compared with healthy smokers and positively correlated with those of VEGF (r=0.64, p<0.05). CONCLUSION:VEGF and VEGFR-2 expressions were up-regulated in CB and increased expression of VEGF was related with HIF-1α. HIF-1α-regulated VEGF overexpression may be a characteristic of chronic bronchitis.
Authors: Kim Hoenderdos; Katharine M Lodge; Robert A Hirst; Cheng Chen; Stefano G C Palazzo; Annette Emerenciana; Charlotte Summers; Adri Angyal; Linsey Porter; Jatinder K Juss; Christopher O'Callaghan; Edwin R Chilvers; Alison M Condliffe Journal: Thorax Date: 2016-08-31 Impact factor: 9.139
Authors: Katja Wagner; Michael Gröger; Oscar McCook; Angelika Scheuerle; Pierre Asfar; Bettina Stahl; Markus Huber-Lang; Anita Ignatius; Birgit Jung; Matthias Duechs; Peter Möller; Michael Georgieff; Enrico Calzia; Peter Radermacher; Florian Wagner Journal: PLoS One Date: 2015-07-30 Impact factor: 3.240