Rita de Cássia Medeiros de Oliveira1, Marcela Beghini2, Cláudia Renata Bibiano Borges2, Polyanna Miranda Alves2, Marcelo Sivieri de Araújo1, Sanívia Aparecida de Lima Pereira3, Virmondes Rodrigues2, Denise Bertulucci Rocha Rodrigues4. 1. Laboratory of Biopathology and Molecular Biology, University of Uberaba (UNIUBE), Uberaba, Minas Gerais, Brazil. 2. Federal University of Triângulo Mineiro (UFTM/CEFORES), Uberaba, Minas Gerais, Brazil. 3. Laboratory of Biopathology and Molecular Biology, University of Uberaba (UNIUBE), Uberaba, Minas Gerais, Brazil; Federal University of Triângulo Mineiro (UFTM/CEFORES), Uberaba, Minas Gerais, Brazil. 4. Laboratory of Biopathology and Molecular Biology, University of Uberaba (UNIUBE), Uberaba, Minas Gerais, Brazil; Federal University of Triângulo Mineiro (UFTM/CEFORES), Uberaba, Minas Gerais, Brazil. Electronic address: denise.rodrigues@uniube.br.
Abstract
INTRODUCTION: Cysts and periapical granulomas are inflammatory reactions that develop in response to periapical infection by microbial species in dental root canal. It is known that toll-like receptors (TLRs) are pathogen recognition molecules and that galectins are lectins that can be associated with the inflammatory process, stimulating or inhibiting the immune system. The objective of this study was to evaluate the in situ expression of TLRs and galectins in radicular cysts and periapical granulomas. METHODS: We analyzed 62 cases (30 radicular cysts, 27 periapical granulomas, and 5 control cases). Indirect immunohistochemistry was used to evaluate the expression of TLRs (TRL-2 and TLR-4) and galectins (Gal-3 and Gal-9). RESULTS: The expression of Gal-3 and Gal-9 was significantly higher in periapical granulomas and radicular cysts than in the control group. Similarly, both Gal-3 and Gal-9 were expressed significantly more in periapical granulomas than in radicular cysts. The expression of TLR-2 was significantly higher in periapical granulomas and radicular cysts than in the control group, and it was also significantly higher in radicular cysts with sinus tract than in the cases without sinus tract. Furthermore, the expression of TLR-4 was significantly higher in the cases of periapical granulomas with sinus tract than in the cases without sinus tract. CONCLUSIONS: Gal-3/Gal-9 and TLR-2/TLR-4 expression in the periapical granulomas and radicular cysts is associated with reactive periapical inflammation. Pathobiology of periapical disease is a very complex interplay of many bioactive molecules involved in immunoinflammatory responses. Up-regulation of these bioactive molecules might be an important modulator of inflammatory periapical lesions.
INTRODUCTION: Cysts and periapical granulomas are inflammatory reactions that develop in response to periapical infection by microbial species in dental root canal. It is known that toll-like receptors (TLRs) are pathogen recognition molecules and that galectins are lectins that can be associated with the inflammatory process, stimulating or inhibiting the immune system. The objective of this study was to evaluate the in situ expression of TLRs and galectins in radicular cysts and periapical granulomas. METHODS: We analyzed 62 cases (30 radicular cysts, 27 periapical granulomas, and 5 control cases). Indirect immunohistochemistry was used to evaluate the expression of TLRs (TRL-2 and TLR-4) and galectins (Gal-3 and Gal-9). RESULTS: The expression of Gal-3 and Gal-9 was significantly higher in periapical granulomas and radicular cysts than in the control group. Similarly, both Gal-3 and Gal-9 were expressed significantly more in periapical granulomas than in radicular cysts. The expression of TLR-2 was significantly higher in periapical granulomas and radicular cysts than in the control group, and it was also significantly higher in radicular cysts with sinus tract than in the cases without sinus tract. Furthermore, the expression of TLR-4 was significantly higher in the cases of periapical granulomas with sinus tract than in the cases without sinus tract. CONCLUSIONS:Gal-3/Gal-9 and TLR-2/TLR-4 expression in the periapical granulomas and radicular cysts is associated with reactive periapical inflammation. Pathobiology of periapical disease is a very complex interplay of many bioactive molecules involved in immunoinflammatory responses. Up-regulation of these bioactive molecules might be an important modulator of inflammatory periapical lesions.
Authors: Daniela A Brandini; Aline S Takamiya; Pari Thakkar; Samantha Schaller; Rani Rahat; Afsar R Naqvi Journal: Rev Med Virol Date: 2021-03-01 Impact factor: 11.043
Authors: Milica Velickovic; Aleksandar Arsenijevic; Aleksandar Acovic; Dragana Arsenijevic; Jelena Milovanovic; Jelena Dimitrijevic; Zeljko Todorovic; Marija Milovanovic; Tatjana Kanjevac; Nebojsa Arsenijevic Journal: Front Pharmacol Date: 2021-03-19 Impact factor: 5.810
Authors: R Leonardi; R E Perrotta; C Loreto; G Musumeci; S Crimi; J N Dos Santos; M C Rusu; P Bufo; E Barbato; G Pannone Journal: Eur J Histochem Date: 2015-10-26 Impact factor: 3.188