BACKGROUND: Rectal sexually transmitted infections (STIs) may increase HIV susceptibility in men who have sex with men (MSM), and Chlamydia trachomatis is prevalent among HIV-positive MSM. To study STIs and HIV infection in MSM, we first evaluated whether cynomolgus macaques can sustain both C. trachomatis and SHIVSF162p3 infections. METHODS: Four SHIVSF162p3 -positive male cynomolgus macaques were used (n = 3 rectally inoculated with 10(6) IFU; n = 1 control). Systemic and rectal SHIV RNA levels and cytokines were measured by real-time PCR and Luminex assays, respectively. RESULTS: Macaques were successfully Chlamydia infected. Rectal SHIV shedding (P = 0.02 χ(2) ) and levels of G-CSF, IL-1ra, IL-6, IL-8, IFN-γ, and TNF-α (P ≤ 0.01, Mann-Whitney) in rectal secretions increased following infection. CONCLUSIONS: These pilot data successfully demonstrate rectal C. trachomatis-SHIV coinfection in cynomolgus macaques and suggest the feasibility of a rectal C. trachomatis model for SHIV susceptibility and biomedical prevention studies in the context of rectal STIs.
BACKGROUND: Rectal sexually transmitted infections (STIs) may increase HIV susceptibility in men who have sex with men (MSM), and Chlamydia trachomatis is prevalent among HIV-positive MSM. To study STIs and HIV infection in MSM, we first evaluated whether cynomolgus macaques can sustain both C. trachomatis and SHIVSF162p3 infections. METHODS: Four SHIVSF162p3 -positive male cynomolgus macaques were used (n = 3 rectally inoculated with 10(6) IFU; n = 1 control). Systemic and rectal SHIV RNA levels and cytokines were measured by real-time PCR and Luminex assays, respectively. RESULTS: Macaques were successfully Chlamydia infected. Rectal SHIV shedding (P = 0.02 χ(2) ) and levels of G-CSF, IL-1ra, IL-6, IL-8, IFN-γ, and TNF-α (P ≤ 0.01, Mann-Whitney) in rectal secretions increased following infection. CONCLUSIONS: These pilot data successfully demonstrate rectal C. trachomatis-SHIV coinfection in cynomolgus macaques and suggest the feasibility of a rectal C. trachomatis model for SHIV susceptibility and biomedical prevention studies in the context of rectal STIs.
Authors: Ryan Colby Passaro; Eddy R Segura; Amaya Perez-Brumer; Jeanne Cabeza; Silvia M Montano; Jordan E Lake; Jorge Sanchez; Javier R Lama; Jesse L Clark Journal: Sex Transm Dis Date: 2018-09 Impact factor: 2.830
Authors: Sundaram Ajay Vishwanathan; Rachael D Aubert; Monica R Morris; Chunxia Zhao; Christi Philips; George M Khalil; Frank Deyounks; Kristen Kelley; Jana M Ritter; C Y Chen; Ellen N Kersh; Janet M McNicholl Journal: Sex Transm Dis Date: 2017-09 Impact factor: 2.830
Authors: Sundaram A Vishwanathan; Monica R Morris; Richard J Wolitski; Wei Luo; Charles E Rose; Dianna M Blau; Theodros Tsegaye; Sherif R Zaki; David A Garber; Leecresia T Jenkins; Tara C Henning; Dorothy L Patton; R Michael Hendry; Janet M McNicholl; Ellen N Kersh Journal: PLoS One Date: 2015-04-08 Impact factor: 3.240