Inhibition of gamete formation by cycloheximide in pollen tubes of Impatients balsamina.

Cycloheximide (CH) inhibited the division of generative cell in pollen cultures of Impatiens balsamina in concentrations of 1-200 μg/ml without affecting pollen germination or pollen tube growth. This inhibition was reversible up to 3 h in hanging drop cultures and up to 6 h in test tube cultures by replacement of the medium with a CH-free one.