BACKGROUND: Recent studies have shown that endothelial progenitor cells (EPCs) contribute to lung repair after lipopolysaccharide (LPS)-induced lung injury and infusion of LPS decreased early EPCs in human peripheral blood. However, the effects of LPS on endothelial colony-forming cells (ECFCs) remain to be determined. OBJECTIVE: To investigate possible effects of LPS on the functional activity of ECFCs. METHODS: ECFCs were isolated from human umbilical cord blood and characterized. ECFCs at passages 3-5 were treated for 24 h with either LPS or vehicle control. Their viability, migration and in vitro vasculogenesis activity were assayed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, modified Boyden chamber and in vitro angiogenesis assays, respectively. ECFC adhesion was assessed by replating cells on fibronectin-coated dishes and subsequent counting of adherent cells. RESULTS: Incubation with LPS dose-dependently inhibited the viable, migratory, adhesive and in vitro vasculogenesis capacity of ECFCs. CONCLUSION: LPS impaired the functional activity of ECFCs.
BACKGROUND: Recent studies have shown that endothelial progenitor cells (EPCs) contribute to lung repair after lipopolysaccharide (LPS)-induced lung injury and infusion of LPS decreased early EPCs in human peripheral blood. However, the effects of LPS on endothelial colony-forming cells (ECFCs) remain to be determined. OBJECTIVE: To investigate possible effects of LPS on the functional activity of ECFCs. METHODS: ECFCs were isolated from human umbilical cord blood and characterized. ECFCs at passages 3-5 were treated for 24 h with either LPS or vehicle control. Their viability, migration and in vitro vasculogenesis activity were assayed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, modified Boyden chamber and in vitro angiogenesis assays, respectively. ECFC adhesion was assessed by replating cells on fibronectin-coated dishes and subsequent counting of adherent cells. RESULTS: Incubation with LPS dose-dependently inhibited the viable, migratory, adhesive and in vitro vasculogenesis capacity of ECFCs. CONCLUSION: LPS impaired the functional activity of ECFCs.
Authors: Laura Giusti; Morena Gabriele; Giuseppe Penno; Monia Garofolo; Vincenzo Longo; Stefano Del Prato; Daniela Lucchesi; Laura Pucci Journal: Oxid Med Cell Longev Date: 2017-03-13 Impact factor: 6.543
Authors: Tobias Kisch; Caroline Weber; Daniel H Rapoport; Charli Kruse; Sandra Schumann; Felix H Stang; Frank Siemers; Anna E Matthießen Journal: PLoS One Date: 2015-11-13 Impact factor: 3.240