Rosa Estrada-Reyes1, C López-Rubalcava2, Octavio Alberto Ferreyra-Cruz1, Ana María Dorantes-Barrón1, G Heinze3, Julia Moreno Aguilar1, Mariano Martínez-Vázquez4. 1. Laboratorio de Fitofarmacología, Dirección de Investigaciones en Neurociencias del Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Calzada México Xochimilco 101, Sn. Lorenzo Huipulco, México, D.F. 14370, México. 2. Departamento de Farmacobiología, Centro de Investigación y de Estudios Avanzados del IPN. Calzada de los Tenorios 235, Col Granjas Coapa CP, 14330 México, D.F., Mexico. 3. Departamento de Psiquiatría y Salud Mental, Facultad de Medicina, Universidad Nacional Autónoma de México, Edificio F s/n, Circuito Interior y Cerro del Agua, Ciudad Universitaria, Coyoacán, México, D.F. 04510, Mexico. 4. Instituto de Química, Universidad Nacional Autónoma de México, Coyoacán 04510, México, D.F., Mexico.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Agastache mexicana subspecies mexicana (Amm) and xolocotziana (Amx) are used in Mexican traditional medicine to relief cultural affiliation syndromes known as "susto" or "espanto", for "nervous" condition, and as a sleep aid. Despite its intensive use, neuropharmacological studies are scarce, and the chemical composition of the aqueous extracts has not been described. Aims of the study are: (1) To analyze the chemical composition of aqueous extracts from aerial parts of Amm and Amx. (2) To evaluate the anxiolytic-like, sedative, antidepressant-like effects. (3) Analyze the general toxic effects of different doses. MATERIALS AND METHODS: Anxiolytic-like and sedative effects were measured in the avoidance exploratory behavior, burying behavior and the hole-board tests. The antidepressant-like actions were studied in the forced swimming and tail suspension tests. Finally, general activity and motor coordination disturbances were evaluated in the open field, inverted screen and rota-rod tests. The acute toxicity of Amm and Amx was determined by calculating their LD50 (mean lethal dose). The chemical analyses were performed employing chromatographic, photometric and HPLC-ESI-MS techniques. RESULTS: Low doses of Amm and Amx (0.1σ1.0mg/kg) induced anxiolytic-like actions; while higher doses (over 10mg/kg) induced sedation and reduced the locomotor activity, exerting a general inhibition in the central nervous system (CNS). CONCLUSIONS: Results support the use of Amm and Amx in traditional medicine as tranquilizers and sleep inducers. Additionally, this paper contributes to the knowledge of the chemical composition of the aqueous extracts of these plants.
ETHNOPHARMACOLOGICAL RELEVANCE: Agastache mexicana subspecies mexicana (Amm) and xolocotziana (Amx) are used in Mexican traditional medicine to relief cultural affiliation syndromes known as "susto" or "espanto", for "nervous" condition, and as a sleep aid. Despite its intensive use, neuropharmacological studies are scarce, and the chemical composition of the aqueous extracts has not been described. Aims of the study are: (1) To analyze the chemical composition of aqueous extracts from aerial parts of Amm and Amx. (2) To evaluate the anxiolytic-like, sedative, antidepressant-like effects. (3) Analyze the general toxic effects of different doses. MATERIALS AND METHODS: Anxiolytic-like and sedative effects were measured in the avoidance exploratory behavior, burying behavior and the hole-board tests. The antidepressant-like actions were studied in the forced swimming and tail suspension tests. Finally, general activity and motor coordination disturbances were evaluated in the open field, inverted screen and rota-rod tests. The acute toxicity of Amm and Amx was determined by calculating their LD50 (mean lethal dose). The chemical analyses were performed employing chromatographic, photometric and HPLC-ESI-MS techniques. RESULTS: Low doses of Amm and Amx (0.1σ1.0mg/kg) induced anxiolytic-like actions; while higher doses (over 10mg/kg) induced sedation and reduced the locomotor activity, exerting a general inhibition in the central nervous system (CNS). CONCLUSIONS: Results support the use of Amm and Amx in traditional medicine as tranquilizers and sleep inducers. Additionally, this paper contributes to the knowledge of the chemical composition of the aqueous extracts of these plants.
Authors: Guadalupe Carrillo-Galván; Robert Bye; Luis E Eguiarte; Sol Cristians; Pablo Pérez-López; Francisco Vergara-Silva; Mario Luna-Cavazos Journal: J Ethnobiol Ethnomed Date: 2020-05-01 Impact factor: 2.733
Authors: Rosa Estrada-Reyes; Marcela Valdés-Tovar; Daniel Arrieta-Baez; Ana María Dorantes-Barrón; Daniel Quero-Chávez; Héctor Solís-Chagoyán; Jesús Argueta; Margarita L Dubocovich; Gloria Benítez-King Journal: Int J Mol Sci Date: 2018-08-03 Impact factor: 5.923
Authors: Alberto Hernandez-Leon; Gabriel Fernando Moreno-Pérez; Martha Martínez-Gordillo; Eva Aguirre-Hernández; María Guadalupe Valle-Dorado; María Irene Díaz-Reval; María Eva González-Trujano; Francisco Pellicer Journal: Molecules Date: 2021-12-16 Impact factor: 4.411