BACKGROUND: It has been indicated that oxidative damage contributes to secondary brain injury in both ischemic and hemorrhagic stroke patients. Collagen is a major component of the extracellular matrix, and prolidase plays a role in collagen synthesis. The aim of this study was to evaluate the serum prolidase activity, nitric oxide (NO) levels, total antioxidant capacity (TAC) and total oxidant status (TOS) in patients with acute hemorrhagic stroke. METHODS: Twenty-five patients with acute hemorrhagic stroke and 25 controls were enrolled. Serum prolidase activity, catalase activity, NO levels, TAC and TOS were measured spectrophotometrically. Oxidative stress index (OSI) was calculated. RESULTS: Serum TAC levels and catalase activity were significantly lower in acute hemorrhagic stroke patients than controls (both, p < 0.001), while NO levels, TOS levels, OSI values and prolidase activity were significantly higher (all, p < 0.01). When patients with acute hemorrhagic stroke were divided according to gender, no differences were observed between females and males in respect to serum prolidase enzyme activity, NO levels, TAC levels, TOS levels and OSI values (all, p > 0.05). CONCLUSIONS: Findings from the study suggest an association between increased oxidative stress levels, decreased antioxidant levels and increased prolidase enzyme activity in patients with acute hemorrhagic stroke compared with controls. More studies are needed to elucidate mechanistic pathways on oxidative stress in patients with acute hemorrhagic stroke.
BACKGROUND: It has been indicated that oxidative damage contributes to secondary brain injury in both ischemic and hemorrhagic strokepatients. Collagen is a major component of the extracellular matrix, and prolidase plays a role in collagen synthesis. The aim of this study was to evaluate the serum prolidase activity, nitric oxide (NO) levels, total antioxidant capacity (TAC) and total oxidant status (TOS) in patients with acute hemorrhagic stroke. METHODS: Twenty-five patients with acute hemorrhagic stroke and 25 controls were enrolled. Serum prolidase activity, catalase activity, NO levels, TAC and TOS were measured spectrophotometrically. Oxidative stress index (OSI) was calculated. RESULTS: Serum TAC levels and catalase activity were significantly lower in acute hemorrhagic strokepatients than controls (both, p < 0.001), while NO levels, TOS levels, OSI values and prolidase activity were significantly higher (all, p < 0.01). When patients with acute hemorrhagic stroke were divided according to gender, no differences were observed between females and males in respect to serum prolidase enzyme activity, NO levels, TAC levels, TOS levels and OSI values (all, p > 0.05). CONCLUSIONS: Findings from the study suggest an association between increased oxidative stress levels, decreased antioxidant levels and increased prolidase enzyme activity in patients with acute hemorrhagic stroke compared with controls. More studies are needed to elucidate mechanistic pathways on oxidative stress in patients with acute hemorrhagic stroke.
Authors: Nurettin Karacan; Mustafa Çalik; Halil Kazanasmaz; Özlem Ethemoğlu; Ahmet Güzelçiçek; Sedat Yaşin; Hasan Kandemir; Emre Çeçen Journal: Ann Indian Acad Neurol Date: 2020-06-29 Impact factor: 1.383
Authors: Julia Masomi-Bornwasser; Elena Kurz; Christina Frenz; Jan Schmitt; Dominik M A Wesp; Jochem König; Johannes Lotz; Florian Ringel; Thomas Kerz; Harald Krenzlin; Naureen Keric Journal: Biomolecules Date: 2021-11-01
Authors: Marta Serrano-Ponz; Carmen Rodrigo-Gasqué; Eva Siles; Esther Martínez-Lara; Laura Ochoa-Callejero; Alfredo Martínez Journal: Mol Med Rep Date: 2016-03-18 Impact factor: 2.952