Substance P-containing medullary projections to the intermediolateral cell column: identification with retrogradely transported rhodamine-labeled latex microspheres and immunohistochemistry.

Substance P (SP)-containing medullary neurons that project to the intermediolateral cell column (IML) of the rat were studied. Neurons were retrogradely labeled with rhodamine-labeled latex microspheres (RITC-M) injected into the T-3 IML, and SP-immunoreactive neurons were identified with immunocytochemistry. RITC-M labeled cells occurred in the nucleus reticularis paragigantocellular lateralis (RPgcl), adjacent and lateral to the pyramidal tract at the level of the rostral inferior olivary nucleus and extended to the mid-facial nucleus in the medulla. Cells were also labeled caudal to the RPgcl, in the nucleus reticularis ventralis, pars alpha (RVa), rostral to the RVa, in the nucleus reticularis gigantocellularis (RGc), and in the raphe nuclei. SP immunoreactivity (SP-IR) was seen in cells that were also retrogradely labeled. These double-labeled cells were observed in the RPgcl, RVa and the raphe pallidus. These data show that the IML receives SP-neuronal projections from multiple locations in the medulla. The SP-neuronal projections from the RPgcl of the ventral medulla to the IML likely represent one component of the ventral medullary region that influences cardiovascular functions.