Literature DB >> 2445403

Diploid and tetraploid precursors of megakaryocytes in normal human bone marrow detected by immunofluorescence.

D Renner1, H Propp, W Queisser.   

Abstract

A sequential preparation method is described which allows immunological identification, morphological characterization, cytophotometric determination of relative DNA content of the megakaryocyte lineage as well as quantitation of megakaryocyte precursors in human bone marrow aspirates. We compared several monoclonal (anti-GP IIIa and HD 19) and polyclonal (A225, RAHPS) antiplatelet antibodies for immunofluorescent staining. Among the identified cells, a small number of cells showing a diploid and tetraploid DNA content were found which must be regarded as promegakaryoblasts, representing 2.5-4.7% of all megakaryocytes. The heterogenous morphology of these precursors in panoptically stained smears is described.

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Year:  1987        PMID： 2445403     DOI： 10.1007/bf00367464

Source DB:  PubMed          Journal:  Blut        ISSN： 0006-5242


  17 in total

1.  Kinetics of megacaryocyte proliferation.

Authors:  L E FEINENDEGEN; N ODARTCHENKO; H COTTIER; V P BOND
Journal:  Proc Soc Exp Biol Med       Date:  1962-10

2.  Identification of young megakaryocytes by immunofluorescence and cytophotometry.

Authors:  M Mayer; J Schaefer; W Queisser
Journal:  Blut       Date:  1978-11-13

3.  Fluorometric measurement of deoxyribonucleic acid in bone marrow cells. The measurement of megakaryocyte deoxyribonucleic acid.

Authors:  S M Weste; D G Penington
Journal:  J Histochem Cytochem       Date:  1972-08       Impact factor: 2.479

Review 4.  Human megakaryocytopoiesis.

Authors:  A M Gewirtz
Journal:  Semin Hematol       Date:  1986-01       Impact factor: 3.851

5.  Binding of platelets to human monocytes: a source of artifacts in the study of the specificity of antileukocyte antibodies.

Authors:  B Perussia; J Jankiewicz; G Trinchieri
Journal:  J Immunol Methods       Date:  1982       Impact factor: 2.303

6.  Immunological study of in vitro maturation of human megakaryocytes.

Authors:  G Vinci; A Tabilio; J F Deschamps; D Van Haeke; A Henri; J Guichard; P Tetteroo; P M Lansdorp; T Hercend; W Vainchenker
Journal:  Br J Haematol       Date:  1984-04       Impact factor: 6.998

7.  Immature Megakaryocytes in the Mouse: Morphology and quantitation by acetylcholinesterase staining.

Authors:  M W Long; N Williams
Journal:  Blood       Date:  1981-11       Impact factor: 22.113

8.  Polyploidization of megakaryocytes in normal humans, in patients with idiopathic thrombocytopenia and with pernicious anaemia.

Authors:  U Queisser; W Queisser; B Spiertz
Journal:  Br J Haematol       Date:  1971-05       Impact factor: 6.998

9.  Two monoclonal antiplatelet antibodies as markers of human megakaryocyte maturation: immunofluorescent staining and platelet peroxidase detection in megakaryocyte colonies and in in vivo cells from normal and leukemic patients.

Authors:  W Vainchenker; J F Deschamps; J M Bastin; J Guichard; M Titeux; J Breton-Gorius; A J McMichael
Journal:  Blood       Date:  1982-03       Impact factor: 22.113

10.  Human megakaryocytes. II. Expression of platelet proteins in early marrow megakaryocytes.

Authors:  E M Rabellino; R B Levene; L L Leung; R L Nachman
Journal:  J Exp Med       Date:  1981-07-01       Impact factor: 14.307
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  2 in total

1.  Histochemical study on the maturation of human megakaryocytes using microfluorometry.

Authors:  N Maruo; Y Kobayashi; H Horiuchi; M Kondo; S Fujita
Journal:  Histochemistry       Date:  1992

Review 2.  Megakaryocytopoiesis in haematological disorders: diagnostic features of bone marrow biopsies. An overview.

Authors:  J Thiele; R Fischer
Journal:  Virchows Arch A Pathol Anat Histopathol       Date:  1991
  2 in total

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