Ahmet Özgür Yeniel1, Oytun Erbas2, Ahmet Mete Ergenoglu3, Huseyin Aktug4, Dilek Taskiran2, Nuri Yildirim3, Murat Ulukus3. 1. Department of Obstetrics and Gynecology, Ege University, Bornova, Izmir, Turkey. Electronic address: drayeniel@hotmail.com. 2. Department of Physiology, Ege University, 35100 Bornova, Izmir, Turkey. 3. Department of Obstetrics and Gynecology, Ege University, Bornova, Izmir, Turkey. 4. Department of Histology, Ege University, 35100 Bornova, Izmir, Turkey.
Abstract
OBJECTIVE: To determine the effects of oxytocin (OT) on surgically induced endometriosis in a rat model. STUDY DESIGN: Twelve female Sprague-Dawley rats were included. After the implantation and establishment of autologous endometrium onto the abdominal wall peritoneum, the rats were randomly divided into two groups, treated with intramuscular oxytocin (OT group, 160μgkg/day, n=6) or isotonic NaCl solution (control group, 1mLkg/day, n=6) for 28 days. To evaluate the therapeutic effects of OT, the explant volumes were calculated and the levels of vascular endothelial growth factor (VEGF), monocyte chemotactic protein-1, and TNF-α were measured in plasma and peritoneal fluid. Endometriotic explants were examined histologically by semiquantitative analysis. RESULTS: After treatment, the mean endometriotic explant volume was decreased in the OT group (p=0.016). The histopathological score and VEGF immunoexpression of endometriotic explants were significantly lower in the OT group (p=0.007) than in controls (p=0.000). Inflammatory cytokine levels in plasma and peritoneal fluid were considerably decreased in the OT group. Moreover, TUNEL immunohistochemistry clearly demonstrated more apoptotic changes in the mononuclear cells of the OT group compared with controls. CONCLUSION: We suggest that oxytocin might be considered as a potential candidate therapeutic agent for endometriosis.
OBJECTIVE: To determine the effects of oxytocin (OT) on surgically induced endometriosis in a rat model. STUDY DESIGN: Twelve female Sprague-Dawley rats were included. After the implantation and establishment of autologous endometrium onto the abdominal wall peritoneum, the rats were randomly divided into two groups, treated with intramuscular oxytocin (OT group, 160μgkg/day, n=6) or isotonic NaCl solution (control group, 1mLkg/day, n=6) for 28 days. To evaluate the therapeutic effects of OT, the explant volumes were calculated and the levels of vascular endothelial growth factor (VEGF), monocyte chemotactic protein-1, and TNF-α were measured in plasma and peritoneal fluid. Endometriotic explants were examined histologically by semiquantitative analysis. RESULTS: After treatment, the mean endometriotic explant volume was decreased in the OT group (p=0.016). The histopathological score and VEGF immunoexpression of endometriotic explants were significantly lower in the OT group (p=0.007) than in controls (p=0.000). Inflammatory cytokine levels in plasma and peritoneal fluid were considerably decreased in the OT group. Moreover, TUNEL immunohistochemistry clearly demonstrated more apoptotic changes in the mononuclear cells of the OT group compared with controls. CONCLUSION: We suggest that oxytocin might be considered as a potential candidate therapeutic agent for endometriosis.
Authors: Michaela G Cuneo; Angela Szeto; Andrew Schrepf; Ellen M Kinner; Benjamin I Schachner; Raisa Ahmed; Premal H Thaker; Michael Goodheart; David Bender; Steve W Cole; Philip M McCabe; Anil K Sood; Susan K Lutgendorf; Armando J Mendez Journal: Psychoneuroendocrinology Date: 2019-04-06 Impact factor: 4.905
Authors: Luis Fernando Bittar; Letícia Queiroz da Silva; Fernanda Loureiro de Andrade Orsi; Kiara Cristina Senger Zapponi; Bruna de Moraes Mazetto; Erich Vinícius de Paula; Silmara Aparecida de Lima Montalvão; Joyce Maria Annichino-Bizzacchi Journal: PLoS One Date: 2020-01-16 Impact factor: 3.240