BACKGROUND: Unlike cervical, anogenital and oropharyngeal cancers, where high-risk human papillomavirus (hrHPV) has long been known to play a major role, a causative link between HPV and lung cancer has been investigated for decades with discrepant results. METHODS: Lung cancer patients eligible for surgical treatment were tested for the presence of HPV-DNA in excised, fresh frozen lung tumor tissue. Patients that tested positive were further examined for the presence of HPV-DNA in adjacent normal lung parenchyma. HPV detection and genotyping was performed using a polymerase chain reaction (PCR)-based approach and allowed the typing of 13 "high-risk"-HPV-types and 2 "low-risk"-HPV-types. RESULTS: Of the 334 tumor-DNA samples tested, 13 (3.9%) showed presence of HPV-DNA, of which 12 were of a high-risk HPV type (16, 33, 66). In those tested positive, HPV-DNA was not found in adjacent normal lung tissue. No correlation with smoking or EGFR/KRAS mutation status was seen, and only one of 84 squamous cell carcinomas was HPV-positive. CONCLUSION: We conclude that HPV is rarely associated with lung cancer in a Northern European population and in those tested positive, more functional studies are required to determine the role HPV plays in lung cancer oncogenesis.
BACKGROUND: Unlike cervical, anogenital and oropharyngeal cancers, where high-risk human papillomavirus (hrHPV) has long been known to play a major role, a causative link between HPV and lung cancer has been investigated for decades with discrepant results. METHODS:Lung cancerpatients eligible for surgical treatment were tested for the presence of HPV-DNA in excised, fresh frozen lung tumor tissue. Patients that tested positive were further examined for the presence of HPV-DNA in adjacent normal lung parenchyma. HPV detection and genotyping was performed using a polymerase chain reaction (PCR)-based approach and allowed the typing of 13 "high-risk"-HPV-types and 2 "low-risk"-HPV-types. RESULTS: Of the 334 tumor-DNA samples tested, 13 (3.9%) showed presence of HPV-DNA, of which 12 were of a high-risk HPV type (16, 33, 66). In those tested positive, HPV-DNA was not found in adjacent normal lung tissue. No correlation with smoking or EGFR/KRAS mutation status was seen, and only one of 84 squamous cell carcinomas was HPV-positive. CONCLUSION: We conclude that HPV is rarely associated with lung cancer in a Northern European population and in those tested positive, more functional studies are required to determine the role HPV plays in lung cancer oncogenesis.
Authors: L Rojas; D Mayorga; A Ruiz-Patiño; J Rodríguez; A F Cardona; P Archila; J Avila; M Bravo; L Ricaurte; C Sotelo; O Arrieta; Z L Zatarain-Barrón; H Carranza; J Otero; C Vargas; F Barrón; L Corrales; C Martín; G Recondo; L E Pino; M A Bermudez; T Gamez; C Ordoñez-Reyes; J E García-Robledo; V C de Lima; H Freitas; N Santoyo; U Malapelle; A Russo; C Rolfo; R Rosell Journal: ESMO Open Date: 2022-06-23
Authors: Estela Maria Silva; Vânia Sammartino Mariano; Paula Roberta Aguiar Pastrez; Miguel Cordoba Pinto; Emily Montosa Nunes; Laura Sichero; Luisa Lina Villa; Cristovam Scapulatempo-Neto; Kari Juhani Syrjanen; Adhemar Longatto-Filho Journal: Infect Agent Cancer Date: 2019-08-02 Impact factor: 2.965