Cheng-Qin Wang1, Feng-Gang Xiang2, Yu-Jun Li3, Xiao-Ming Xing4, Ning Wang1, Jing-Hua Chi1, Wen-Juan Yu4. 1. Lecturer, Department of Pathology, Medical College of Qingdao University, Qingdao, Shandong, China. 2. Lecturer, Department of Pathology, Medical College of Qingdao University, Qingdao, Shandong, China. Electronic address: xiangfenggang@163.com. 3. Professor and Attending Physician, Department of Pathology, Affiliated Hospital of the Medical College of Qingdao University, Qingdao, Shandong, China. Electronic address: wcq0879@126.com. 4. Professor and Attending Physician, Department of Pathology, Affiliated Hospital of the Medical College of Qingdao University, Qingdao, Shandong, China.
Abstract
OBJECTIVE: Mitotic centromere-associated kinesin (MCAK) is a microtubule depolymerase indispensable for microtubule binding during spindle formation. The purpose of this study was to investigate the association of MCAK expression with squamous cell carcinoma of the oral tongue (SCCOT). STUDY DESIGN: Immunohistochemistry was used in 47 cases of SCCOT. Determination of proliferation and migratory capabilities was performed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and Transwell chamber assay, respectively, on cells from the human tongue squamous cell carcinoma cell line Tca8113 that were transfected with MCAK small interfering RNA (siRNA). RESULTS: MCAK expression level in oral tongue cancer tissue is significantly higher (P < .01) than that of corresponding normal tissue. In addition, high expression of MCAK is significantly associated with lymph node metastasis (P < .05) and tumor staging (P < .01). Moreover, gene silencing of MCAK suppresses proliferation and migration of Tca8113 cells (P < .05; P < .01). CONCLUSIONS: The expression of MCAK may be associated with the progression of SCCOT. Crown
OBJECTIVE:Mitotic centromere-associated kinesin (MCAK) is a microtubule depolymerase indispensable for microtubule binding during spindle formation. The purpose of this study was to investigate the association of MCAK expression with squamous cell carcinoma of the oral tongue (SCCOT). STUDY DESIGN: Immunohistochemistry was used in 47 cases of SCCOT. Determination of proliferation and migratory capabilities was performed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and Transwell chamber assay, respectively, on cells from the human tongue squamous cell carcinoma cell line Tca8113 that were transfected with MCAK small interfering RNA (siRNA). RESULTS:MCAK expression level in oral tongue cancer tissue is significantly higher (P < .01) than that of corresponding normal tissue. In addition, high expression of MCAK is significantly associated with lymph node metastasis (P < .05) and tumor staging (P < .01). Moreover, gene silencing of MCAK suppresses proliferation and migration of Tca8113 cells (P < .05; P < .01). CONCLUSIONS: The expression of MCAK may be associated with the progression of SCCOT. Crown