| Literature DB >> 24440802 |
Hamid Kalantari1, Nasrin Motamed2, Anahita Mohseni Meybodi3, Ali Jabbari Arfaie4, Hossein Baharvand5, Hamid Gourabi6.
Abstract
Low doses of ionizing radiation may induce an adaptive mechanism which protects embryonic stem cells against higher doses, a phenomenon which was reported previously for somatic cells. In this study, a possible adaptive response (AR) was evaluated by measuring cell survival (MTT assay) and chromosomal aberrations (micronucleus assay). Thymidine-synchronized mouse embryonic stem cells (mESCs) were exposed to 2.5, 3.7, or 5cGy (60)Co γ-rays and, after 5h challenged by a dose of 150cGy. mESCs pre-irradiated at 2.5cGy showed an adaptive response.Entities:
Keywords: Adaptive response; Cytochalasin-B; Ionizing radiation; MTT assay; Micronucleus assay; Mouse embryonic stem cell
Mesh：
Year: 2014 PMID： 24440802 DOI： 10.1016/j.mrgentox.2014.01.002
Source DB: PubMed Journal: Mutat Res Genet Toxicol Environ Mutagen ISSN： 1383-5718 Impact factor: 2.873