Jesus Igor Iruretagoyena1, Anna Gonzalez-Tendero2, Patricia Garcia-Canadilla3, Ivan Amat-Roldan2, Iratxe Torre2, Alfons Nadal4, Fatima Crispi5, Eduard Gratacos6. 1. Fetal and Perinatal Medicine Research Group, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain; Department of Maternal-Fetal Medicine, Hospital Clinic, Universitat de Barcelona, Barcelona, Spain. 2. Fetal and Perinatal Medicine Research Group, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain. 3. Fetal and Perinatal Medicine Research Group, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain; Physense, Departament de Tecnologies de la Informació i les Comunicacions (DTIC), Universitat Pompeu Fabra, Barcelona, Spain. 4. Department of Pathology, Hospital Clinic, Universitat de Barcelona, Barcelona, Spain. 5. Fetal and Perinatal Medicine Research Group, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain; Department of Maternal-Fetal Medicine, Hospital Clinic, Universitat de Barcelona, Barcelona, Spain; Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Barcelona, Spain. 6. Fetal and Perinatal Medicine Research Group, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain; Department of Maternal-Fetal Medicine, Hospital Clinic, Universitat de Barcelona, Barcelona, Spain; Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Barcelona, Spain. Electronic address: gratacos@clinic.ub.es.
Abstract
OBJECTIVE: The purpose of this study was to assess whether abnormal cardiac function in human fetuses with intrauterine growth restriction (IUGR) is associated with ultrastructural differences in the cardiomyocyte sarcomere. STUDY DESIGN: Nine severe early-onset IUGR fetuses and 9 normally grown fetuses (appropriate growth for gestational age) who died in the perinatal period were included prospectively. Cardiac function was assessed by echocardiography and levels of B-type natriuretic peptide and troponin-I. Heart sections were imaged by second harmonic generation microscopy, which allowed unstained visualization of cardiomyocyte's sarcomere length. RESULTS: Echocardiographic and biochemical markers showed signs of severe cardiac dysfunction in IUGR fetuses. Second harmonic generation microscopy demonstrated a significantly shorter sarcomere length in IUGR as compared with appropriate growth for gestational age fetuses. CONCLUSION: IUGR is associated with changes in the cardiomyocyte contractile machinery in the form of shorter sarcomere length, which could help to explain the cardiac dysfunction previously documented in IUGR.
OBJECTIVE: The purpose of this study was to assess whether abnormal cardiac function in human fetuses with intrauterine growth restriction (IUGR) is associated with ultrastructural differences in the cardiomyocyte sarcomere. STUDY DESIGN: Nine severe early-onset IUGR fetuses and 9 normally grown fetuses (appropriate growth for gestational age) who died in the perinatal period were included prospectively. Cardiac function was assessed by echocardiography and levels of B-type natriuretic peptide and troponin-I. Heart sections were imaged by second harmonic generation microscopy, which allowed unstained visualization of cardiomyocyte's sarcomere length. RESULTS: Echocardiographic and biochemical markers showed signs of severe cardiac dysfunction in IUGR fetuses. Second harmonic generation microscopy demonstrated a significantly shorter sarcomere length in IUGR as compared with appropriate growth for gestational age fetuses. CONCLUSION: IUGR is associated with changes in the cardiomyocyte contractile machinery in the form of shorter sarcomere length, which could help to explain the cardiac dysfunction previously documented in IUGR.
Authors: Anderson H Kuo; Cun Li; Hillary F Huber; Matthias Schwab; Peter W Nathanielsz; Geoffrey D Clarke Journal: J Physiol Date: 2017-05-18 Impact factor: 5.182
Authors: Anderson H Kuo; Cun Li; Jinqi Li; Hillary F Huber; Peter W Nathanielsz; Geoffrey D Clarke Journal: J Physiol Date: 2016-12-17 Impact factor: 5.182
Authors: Iratxe Torre; Anna González-Tendero; Patricia García-Cañadilla; Fátima Crispi; Francisco García-García; Bart Bijnens; Igor Iruretagoyena; Joaquin Dopazo; Ivan Amat-Roldán; Eduard Gratacós Journal: PLoS One Date: 2014-11-17 Impact factor: 3.240