Literature DB >> 2444027

Antigenic variation of porcine transmissible gastroenteritis virus detected by monoclonal antibodies.

T Hohdatsu1, Y Eiguchi, M Tsuchimoto, S Ide, H Yamagishi, M Matumoto.   

Abstract

Mouse myeloma cells (SP2/O) were fused with spleen cells from BALB/c mice immunized with detergent-solubilized antigen of purified virus, and 21 monoclonal (MC) antibodies reactive in enzyme-linked immunosorbent assay with the TO-163 strain of porcine transmissible gastroenteritis (TGE) virus were obtained. Of these MC antibodies, 14, 6 and 1 were IgG1, IgG2a and IgM, respectively. All of the MC antibodies contained light chains of the kappa type. Of these MC antibodies, 8 were found to have neutralization (NT) activity against the TO-163 strain. Comparison of 7 strains of TGE virus by NT tests using our panel of MC antibodies confirmed their close antigenic relationships, but also revealed the occurrence of distinct antigenic differences. These results suggest that there may be at least 6 different epitopes involved in NT reaction on the virion of the TO-163 strain. This notion was confirmed by the competitive binding assay.

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Year:  1987        PMID： 2444027      PMCID： PMC7117515          DOI： 10.1016/0378-1135(87)90003-4

Source DB:  PubMed          Journal:  Vet Microbiol        ISSN： 0378-1135            Impact factor:   3.293


  11 in total

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Journal:  Nature       Date:  1975-08-07       Impact factor: 49.962

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Journal:  J Virol       Date:  1986-03       Impact factor: 5.103

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Journal:  Natl Inst Anim Health Q (Tokyo)       Date:  1975

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Authors:  P K Nakane; A Kawaoi
Journal:  J Histochem Cytochem       Date:  1974-12       Impact factor: 2.479

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Authors:  A D Blann
Journal:  Med Lab Sci       Date:  1979

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Journal:  Natl Inst Anim Health Q (Tokyo)       Date:  1967

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Authors:  J Kimura-Kuroda; K Yasui
Journal:  J Virol       Date:  1983-01       Impact factor: 5.103

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Journal:  Am J Vet Res       Date:  1981-03       Impact factor: 1.156

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Journal:  J Gen Virol       Date:  1986-01       Impact factor: 3.891

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Journal:  Can J Comp Med       Date:  1976-04
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  9 in total

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Authors:  K A Hussain; J Storz; K G Kousoulas
Journal:  Virology       Date:  1991-07       Impact factor: 3.616

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Authors:  R A Simkins; L J Saif; P A Weilnau
Journal:  Arch Virol       Date:  1989       Impact factor: 2.574

3.  Sequence analysis of the partial spike glycoprotein gene of porcine epidemic diarrhea viruses isolated in Korea.

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Journal:  Virus Genes       Date:  2007-04-11       Impact factor: 2.332

4.  A competitive inhibition ELISA for the differentiation of serum antibodies from pigs infected with transmissible gastroenteritis virus (TGEV) or with the TGEV-related porcine respiratory coronavirus.

Authors:  P Callebaut; M B Pensaert; J Hooyberghs
Journal:  Vet Microbiol       Date:  1989-05       Impact factor: 3.293

5.  Antigenic analysis of feline coronaviruses with monoclonal antibodies (MAbs): preparation of MAbs which discriminate between FIPV strain 79-1146 and FECV strain 79-1683.

Authors:  T Hohdatsu; T Sasamoto; S Okada; H Koyama
Journal:  Vet Microbiol       Date:  1991-06       Impact factor: 3.293

6.  Characterization of monoclonal antibodies against feline infectious peritonitis virus type II and antigenic relationship between feline, porcine, and canine coronaviruses.

Authors:  T Hohdatsu; S Okada; H Koyama
Journal:  Arch Virol       Date:  1991       Impact factor: 2.574

7.  Antigenic and biological diversity among transmissible gastroenteritis virus isolates of swine.

Authors:  E M Vaughn; P S Paul
Journal:  Vet Microbiol       Date:  1993-09       Impact factor: 3.293

8.  Partial sequence of the spike glycoprotein gene of transmissible gastroenteritis viruses isolated in Korea.

Authors:  Soo Joung Kim; Jeong Hee Han; Hyuk Moo Kwon
Journal:  Vet Microbiol       Date:  2003-07-17       Impact factor: 3.293

9.  Antigenic and biological comparisons of bovine coronaviruses derived from neonatal calf diarrhea and winter dysentery of adult cattle.

Authors:  H Tsunemitsu; L J Saif
Journal:  Arch Virol       Date:  1995       Impact factor: 2.574
  9 in total

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