| Literature DB >> 24439447 |
Melissa K Deck1, Erica S Anderson2, Rebecca J Buckner3, Georgia Colasante4, Thomas E Davis3, James M Coull2, Benjamin Crystal2, Phyllis Della Latta5, Martin Fuchs2, Deanna Fuller3, Will Harris2, Kevin Hazen6, Lisa L Klimas2, Daniel Lindao4, Michelle C Meltzer2, Margie Morgan7, Janeen Shepard2, Sharon Stevens2, Fann Wu5, Mark J Fiandaca2.
Abstract
The performance of a diagnostic method for detection and identification of Enterococcus spp. directly from positive blood culture was evaluated in a clinical study. The method, Enterococcus QuickFISH BC, is a second-generation peptide nucleic acid (PNA) fluorescence in situ hybridization (FISH) test, which uses a simplified, faster assay procedure. The test uses fluorescently labeled PNA probes targeting 16S rRNA to differentiate Enterococcus faecalis from other Enterococcus spp. by the color of the cellular fluorescence. Three hundred fifty-six routine blood culture samples were tested; only 2 discordant results were recorded. The sensitivities for detection of Enterococcus faecalis and non-faecalis Enterococcus were 100% (106/106) and 97.0% (65/67), respectively, and the combined specificity of the assay was 100%. The combined positive and negative predictive values of the assay were 100% (171/171) and 98.9% (185/187), respectively.Entities:
Keywords: Enterococcus; PNA; QuickFISH; septicemia
Mesh：
Year: 2013 PMID： 24439447 DOI： 10.1016/j.diagmicrobio.2013.12.004
Source DB: PubMed Journal: Diagn Microbiol Infect Dis ISSN： 0732-8893 Impact factor: 2.803